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From 2008.igem.org

Protocols Purification of His_Z_alpha and His_Z_omega Purification of His_A_alpha Testing various hunter/prey combinations Plasmid DNA isolation DNA isolation from agarose gel DNA purification after enzymatic reaction Genomic DNA isolation Preparation of chemocompetent bacteria Preparation of electrocompetent bacteria Chemotransformation Electrotransformation Ligation Blunting Standard concentrations of antibiotics and other supplements Rifampicin test Removing 5' phosphate groups from DNA ends TAXI protocol (Tet+Ap 30+X-Gal+IPTG) Protein concentration measurement (BCA method) DNA digest PCR Polymerase Chain Ligation (PCL) If not stated otherwise, all agarose gels bear Fermentas GeneRuler (100-10000 bp) ladder. PAGE gels bear PageRuler Fermentas Prestained protein ladder.

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