Team Sorbitol:
Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.
Started the following cultures:
DH5a-PBAD30 : to harvest the plasmid to clone out the srl operon
DH5a-pBAD30-srlD: from colony 2 to freeze down for back up
DH5a-pBAD18 - to insert srld and the operon into MG1655, JW3890, RL257
This was done to make chemically competent
Team Fungus:
Ran out gradient PCR products on 1% agarose gel. No bands.
Ran TAQ gradient PCR again in one more tube due to low amplification results using only 1 uL of cDNA template.
Ran PCR out on gel. Only positive control showed up.
Made LB+Amp+XGal plates.
Attempted transformation again.
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