Team:Paris/Modeling/f2

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[[Image:f2.jpg|thumb]] According to previous consideration on pBad, we have
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{{Paris/Menu}}
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<center>[[Image:f2expr.jpg]]</center>
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{{Paris/Header|Method & Algorithm : &#131;2}}
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<center> = act_''pBad'' </center>
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<br>
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so, with the complexation between Arabinose and AraC, and after reducing the equation
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[[Image:f2.jpg|thumb|Specific Plasmid Characterisation for &#131;2]]
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<center>[[Image:f2exprRedu.jpg]]</center>
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According to the characterization plasmid (see right) and to our modeling, in the '''exponential phase of growth''', at the steady state, the experiment would give us
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[[Image:f2expr.jpg|center]]
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{|border="1" style="text-align: center"
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and at steady-state and in the exponential phase of growth, we expect :
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|param
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|signification
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|unit
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|value
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|[expr(pBad)]
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|expression rate of <br> pBad '''with RBS E0032'''
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|nM.s<sup>-1</sup>
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|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 measures
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|-
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|γ<sub>GFP</sub>
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|dilution-degradation rate <br> of GFP(mut3b)
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|s<sup>-1</sup>
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|ln(2)/3600
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|[GFP]
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|GFP concentration at steady-state
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|nM
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|need for 20 measures
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|-
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|(''fluorescence'')
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|value of the observed fluorescence
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|au
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|need for 20 measures
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|-
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|''conversion''
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|conversion ration between <br> fluorescence and concentration
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|nM.au<sup>-1</sup>
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|(1/79.429)
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|}
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<br><br>
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[[Image:Exprpbad.jpg|center]]
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{|border="1" style="text-align: center"
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we use this analytical expression to determine the parameters :
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|param
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|signification <br> corresponding parameters in the [[Team:Paris/Modeling/Oscillations#Resulting_Equations|equations]]
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<div style="text-align: center">
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|unit
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{{Paris/Toggle|Table of Values|Team:Paris/Modeling/More_f2_Table}}
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|value
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</div>
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|-
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|β<sub>bad</sub>
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<div style="text-align: center">
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|production rate of pBad '''with RBS E0032''' <br> not in the system
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{{Paris/Toggle|Algorithms|Team:Paris/Modeling/More_f2_Algo}}
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|nM.s<sup>-1</sup>
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</div>
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|
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That will give us directly &#131;2([arab])
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|(K<sub>bad</sub>/[AraC<sub>tot</sub>]<sup>n<sub>bad</sub></sup>)
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|activation constant of pBad <br> not in the system
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<br>
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|nM<sup>n<sub>bad</sub></sup>
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|
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<center>
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|-
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[[Team:Paris/Modeling/Implementation| <Back - to "Implementation" ]]| <br>
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|n<sub>bad</sub>
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[[Team:Paris/Modeling/Protocol_Of_Characterization| <Back - to "Protocol Of Characterization" ]]|
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|complexation order of pBad<br> not in the system
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</center>
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|no dimension
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|
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|-
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|K<sub>ara</sub>
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|complexation constant Arabinose-AraC <br> not in the system
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|nM<sup>n<sub>ara</sub></sup>
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|
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|-
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|n<sub>ara</sub>
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|complexation order Arabinose-AraC <br> not in the system
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|no dimension
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|
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|}
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Latest revision as of 02:05, 30 October 2008

Method & Algorithm : ƒ2


= act_pBad


Specific Plasmid Characterisation for ƒ2

According to the characterization plasmid (see right) and to our modeling, in the exponential phase of growth, at the steady state, the experiment would give us

F2expr.jpg

and at steady-state and in the exponential phase of growth, we expect :

Exprpbad.jpg

we use this analytical expression to determine the parameters :

↓ Table of Values ↑
↓ Algorithms ↑

That will give us directly ƒ2([arab])


<Back - to "Implementation" |
<Back - to "Protocol Of Characterization" |