Team:Hawaii/Notebook/2008-10-28

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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Ran RE digests on gel=== :<strong> Grace</strong> [[Image:102808REdigest.jpg|right|thumb|EtBr stained 4% agarose gel ra...)
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= Things we did today =
= Things we did today =
== Wetlab work ==
== Wetlab work ==
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===Ran RE digests on gel===
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:<strong> Grace</strong>
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[[Image:102808REdigest.jpg|right|thumb|EtBr stained 4% agarose gel ran at 60V for 3.5 hours. Ten microliters of XbaI/SpeI digested DNA were loaded into each lane.]]
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:* Gel picture for presentation
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===[[Team:Hawaii/Antibiotic_test_for_BB-pRL1383a |Antibiotic test]]===
===[[Team:Hawaii/Antibiotic_test_for_BB-pRL1383a |Antibiotic test]]===
:<strong>Grace</strong>
:<strong>Grace</strong>
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[[Image:102808j33207.jpg|right|thumb|EtBr stained 2% agarose gel ran at 60V for 2 hours. Twenty microliters of HindIII/BamHI digested J33207 were loaded.]]
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:* Ran HindIII/BamHI digested J33207 on gel
:* Ran HindIII/BamHI digested J33207 on gel
:* Extracted from gel
:* Extracted from gel
:* Ligated with HindIII/BamHI digested pRL1383a
:* Ligated with HindIII/BamHI digested pRL1383a
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:* Used 10 &mu;l ligation reaction to transform 100 &mu;l DH5&alpha; MCR cells (from Doug in SC lab)
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:* Used 5 &mu;l ligation reaction to transform 100 &mu;l DH5&alpha; MCR cells (from Doug in SC lab)
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::* Also transformed 25 &mu;l DH5&alpha; MCR with 2 &mu;l BB-1 1025 and 1 &mu;l J33207 each.
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[[Image:PRL-K125820.jpg|right|thumb|Plate of BB-pRL1383a/K125820 transformants under blue light.  Only some of the colonies appear to fluoresce and the smaller colonies do not show well in the picture.]]
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===Checked for Transformants===
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:<strong>Krystle</strong>
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:*BB-pRL1383a/K125820 produced a total of 27 transformants of varying size after incubation for 20 hours at 37<sup>o</sup>C
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===Inoculate for Tri-Parental Conjugation===
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:<strong>Krystle</strong>
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:*Inoculated 10ml cultures of LB with
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:** BB-pRL1383a with sp<sub>100</sub>
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:** BB-pRL1383a/K125820 with sp<sub>100</sub>
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:** RP1 with kan<sub>50</sub>
== Drylab Work ==
== Drylab Work ==
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===Name of Task===
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===Project page===
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:<strong> name of person/people who performed the task</strong>
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:<strong>Grace</strong>
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:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
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:* Wrote up Materials, Methods and Results for Project Part B on team project page.
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:* e.g. read through papers, worked on proposal, etc.
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===Sequence analysis===
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:<strong>Grace </strong>
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:* Same G->C transversion in CAP binding site for BBpRL-1 sequence.
= Discussion =
= Discussion =

Latest revision as of 03:41, 30 October 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Antibiotic test

Grace
  • Ran HindIII/BamHI digested J33207 on gel
  • Extracted from gel
  • Ligated with HindIII/BamHI digested pRL1383a
  • Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)
  • Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each.
Plate of BB-pRL1383a/K125820 transformants under blue light. Only some of the colonies appear to fluoresce and the smaller colonies do not show well in the picture.

Checked for Transformants

Krystle
  • BB-pRL1383a/K125820 produced a total of 27 transformants of varying size after incubation for 20 hours at 37oC

Inoculate for Tri-Parental Conjugation

Krystle
  • Inoculated 10ml cultures of LB with
    • BB-pRL1383a with sp100
    • BB-pRL1383a/K125820 with sp100
    • RP1 with kan50

Drylab Work

Project page

Grace
  • Wrote up Materials, Methods and Results for Project Part B on team project page.

Sequence analysis

Grace
  • Same G->C transversion in CAP binding site for BBpRL-1 sequence.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


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