Team:Hawaii/Parts

From 2008.igem.org

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{| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center"
{| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center"
|-
|-
-
! width="100"|Part type
 
! width="100"|Part number
! width="100"|Part number
 +
! width="350"|Part type
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| '''K125000-K125099'''
| '''K125000-K125099'''
-
| plasmid
+
| align="center"|plasmid
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| '''K125100-K125199'''
| '''K125100-K125199'''
-
| promoter
+
| align="center"|promoter
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| '''K125200-K125299'''
| '''K125200-K125299'''
-
| ribosome binding site
+
| align="center"|ribosome binding site
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| '''K125300-K125499'''
| '''K125300-K125499'''
-
| signal sequences, conjugation sequences, random stuff
+
| align="center"|signal sequences, conjugation sequences, random stuff
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| '''K125500-K125699'''
| '''K125500-K125699'''
-
| protein coding
+
| align="center"|protein coding
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| '''K125700-K125799'''
| '''K125700-K125799'''
-
| transcription terminator
+
| align="center"|transcription terminator
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| '''K125800-K125999'''
| '''K125800-K125999'''
-
| devices
+
| align="center"|devices
|}
|}
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==Parts in process (building)==
==Parts in process (building)==
 +
{| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center"
 +
|-
 +
! width="75"|Part number
 +
! width="250"|Description
 +
! width="75"|Type
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125010 '''K125010''']
 +
| Broad-Host-Range plasmid derived from RSF1010
 +
| plasmid
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125330 '''K125330''']
 +
| P1 lytic replication
 +
|
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125340 '''K125340''']
 +
| oriV
 +
|
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125510 '''K125510''']
 +
| Plasmid replication proteins
 +
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125520 '''K125520''']
 +
| aadA gene
 +
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125800 '''K125800''']
 +
| rep proteins with promoter and rbs
 +
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125830 '''K125830''']
 +
| ''Synechocystis''-specific, nitrate-inducible GFP secretion device under the control of a ''nirA'' promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500])
 +
| device
 +
|}
 +
 +
==Parts submitted==
{| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center"
{| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center"
|-
|-
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|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 '''K125000''']
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 '''K125000''']
-
| pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_B0034 (rbs)
+
| pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (''lac'' promoter+''lacZ'')
| plasmid
| plasmid
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 '''K125100''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125100 '''K125100''']
| nitrate inducible ''nir'' promoter
| nitrate inducible ''nir'' promoter
| regulator
| regulator
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 '''K125300''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125110 '''K125110''']
 +
| nitrate inducible ''nir'' promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030]
 +
| regulator
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125300 '''K125300''']
| cyanobacterial pilA derived amino termninus protein secretion signal sequence
| cyanobacterial pilA derived amino termninus protein secretion signal sequence
| protein coding
| protein coding
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 '''K125310''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 '''K125310''']
| cyanobacterial slr2016 derived amino terminus protein secretion signal sequence
| cyanobacterial slr2016 derived amino terminus protein secretion signal sequence
| protein coding
| protein coding
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 '''K125500''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125500 '''K125500''']
| GFP fusion protein
| GFP fusion protein
 +
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125810 '''K125810''']
 +
| N-terminal slr2016 signal sequence attached to GFP fusion protein (Composite of [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500])
 +
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125820 '''K125820''']
 +
| ''Synechocystis''-specific constitutive GFP secretion device under the control of a ''lac'' promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_I14032 I14032]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0034 B0034][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500])
 +
| device
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125350 '''K125350''']
 +
| oriTr/LacZ
 +
| conjugation screening
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125360 '''K125360''']
 +
| oriT/LacZ
 +
| conjugation screening
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125320 '''K125320''']
 +
| oriT derived from RP4
 +
| origin of transfer
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125020 '''K125020''']
 +
| rep/oriv/aada/orit
 +
| intermediate for RSF1010 derived plasmid
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125530 '''K125530''']
 +
| aadA gene with promoter and rbs
| protein coding
| protein coding
|}
|}
-
 
-
==Parts submitted==
 

Latest revision as of 03:53, 30 October 2008

Contents

Guidelines for naming parts

Part number Part type
K125000-K125099 plasmid
K125100-K125199 promoter
K125200-K125299 ribosome binding site
K125300-K125499 signal sequences, conjugation sequences, random stuff
K125500-K125699 protein coding
K125700-K125799 transcription terminator
K125800-K125999 devices

Parts planned

Parts in process (building)

Part number Description Type
[http://partsregistry.org/wiki/index.php/Part:BBa_K125010 K125010] Broad-Host-Range plasmid derived from RSF1010 plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125330 K125330] P1 lytic replication
[http://partsregistry.org/wiki/index.php/Part:BBa_K125340 K125340] oriV
[http://partsregistry.org/wiki/index.php/Part:BBa_K125510 K125510] Plasmid replication proteins protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125520 K125520] aadA gene protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125800 K125800] rep proteins with promoter and rbs protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125830 K125830] Synechocystis-specific, nitrate-inducible GFP secretion device under the control of a nirA promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) device

Parts submitted

Part number Description Type
[http://partsregistry.org/wiki/index.php/Part:BBa_K125000 K125000] pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (lac promoter+lacZ) plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100] nitrate inducible nir promoter regulator
[http://partsregistry.org/wiki/index.php/Part:BBa_K125110 K125110] nitrate inducible nir promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030] regulator
[http://partsregistry.org/wiki/index.php/Part:BBa_K125300 K125300] cyanobacterial pilA derived amino termninus protein secretion signal sequence protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310] cyanobacterial slr2016 derived amino terminus protein secretion signal sequence protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500] GFP fusion protein protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125810 K125810] N-terminal slr2016 signal sequence attached to GFP fusion protein (Composite of [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125820 K125820] Synechocystis-specific constitutive GFP secretion device under the control of a lac promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_I14032 I14032]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0034 B0034][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) device
[http://partsregistry.org/wiki/index.php/Part:BBa_K125350 K125350] oriTr/LacZ conjugation screening
[http://partsregistry.org/wiki/index.php/Part:BBa_K125360 K125360] oriT/LacZ conjugation screening
[http://partsregistry.org/wiki/index.php/Part:BBa_K125320 K125320] oriT derived from RP4 origin of transfer
[http://partsregistry.org/wiki/index.php/Part:BBa_K125020 K125020] rep/oriv/aada/orit intermediate for RSF1010 derived plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125530 K125530] aadA gene with promoter and rbs protein coding
Retrieved from "http://2008.igem.org/Team:Hawaii/Parts"