Team:Chiba/Calendar-Home/20 August 2008

From 2008.igem.org

(Difference between revisions)

Latest revision as of 05:57, 30 October 2008

>Home | Notebook

19 August 2008 <|> 21 August 2008

Laboratory work

Team:Input

PCR

[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)(1)
[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)(2)
[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)(3)
[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)(4)
[http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)(5)

Sample No.	1	2	3	4	5
DNA tamplate(μL)	1	1	1	1	1
FW primer(μL)	5	5	5	5	5
VR primer(μL)	5	5	5	5	5
dNTPmix(μL)	10	10	10	10	10
thermo pol buffer(μL)	10	10	10	10	10
DNA pol(VENT)(μL)	1	1	1	1	1
dH₂O(μL)	68	68	68	68	68
TOTAL(μL)	100	100	100	100	100

-->Gel Check

Sample No.	1	2	3	4	5
Sample DNA	1	1	1	1	1
loading dye(μL)	1	1	1	1	1
dH₂O(μL)	4	4	4	4	4
Total(μL)	6	6	6	6	6

-->1,2,3,4,5:none

Transformation

[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)-->only one colony

Team:Communication

(18/8)--->Mini prep

[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)

--->Gel Check

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> None

Sample DNA	10
Loading Dye	4
dH₂O	10
TOTAL	24μL

From left;

[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> OK
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007) -> OK
[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006) -> OK

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK

--->Digestion test

[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)

Sample No	1	2	3	4	5	6	7	8
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)	3	-	-	-	3	-	-	-
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)	-	3	-	-	-	3	-	-
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)	-	-	3	-	-	-	3	-
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)	-	-	-	3	-	-	-	3
EcoRI	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1
PstI	-	-	-	-	0.1	0.1	0.1	0.1
Buffer EcoRI	1	1	1	1	1	1	1	1
BSA	1	1	1	1	1	1	1	1
dH₂O	4.9	4.9	4.9	4.9	4.8	4.8	4.8	4.8
TOTAL	10μL	10μL	10μL	10μL	10μL	10μL	10μL	10μL

--->Gel Check

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

(Single Digestion 1~4)

[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK

(Double Digestion 1~4)

[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK

Team:Output

Transformation

[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2007)-->colony
[http://partsregistry.org/Part:BBa_I712019 BBa_I712019](2008)-->no colony
[http://partsregistry.org/Part:BBa_J33202 BBa_J33202](2007)-->colony
[http://partsregistry.org/Part:BBa_E2030 BBa_E2030](2007)-->not so good
YFP(P-GEX)-->colony(Amp)
[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->colony
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)-->colony

@@ Line 1: / Line 1: @@
-'''OUTPUT TEAM'''
+>[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]]
-<br>Transformation
-<br>Luciferase(J52008:2007,2008 I712019:2008)(Amp)
-<br>Lac Z(J33202:2007,2008)(Amp)
-<br>Venus(E2030:2007,2008)(Kam)
-<br>Craig Venus(Amp?Kan?)
-<br>T9002(Amp)
-<br>F2620(Amp)
-<br>luciferase,LacZ,Venus
+[[Team:Chiba/Calendar-Home/19 August 2008|19 August 2008 <]]|[[Team:Chiba/Calendar-Home/21 August 2008|> 21 August 2008]]
-<br>competent cell  50μl
-<br>DNA   1μl
-<br>soc  200μl
-<br>plating 250μl
-<br>Craig Venus,T9002,F2620
+==Laboratory work==
-<br>competent cell  30μl
+===Team:Input===
-<br>DNA  1μl
+'''[[Team:Chiba/protocol/PCR|PCR]]'''
-<br>soc  120μl
+*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)(1)
-<br>plating 20μl
+*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)(2)
+*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)(3)
+*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)(4)
+*[http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)(5)
+<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample No.</td>
+<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td>
+</tr>
+<tr>
+<td>DNA tamplate(&mu;L)</td>
+<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+</tr>
+<tr>
+<td>FW primer(&mu;L)</td>
+<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
+</tr>
+<tr>
+<td>VR primer(&mu;L)</td>
+<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
+</tr>
+<tr>
+<td>dNTPmix(&mu;L)</td>
+<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+</tr>
+<tr>
+<td>thermo pol buffer(&mu;L)</td>
+<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+</tr>
+<tr>
+<td>DNA pol(VENT)(&mu;L)</td>
+<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+</tr>
+<tr>
+<td>dH<sub>2</sub>O(&mu;L)</td>
+<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
+</tr>
+<tr>
+<td>TOTAL(&mu;L)</td>
+<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
+</tr>
+</table>
-<br>result
+-->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
-:成功→Luciferase(J52008:2007),Lac Z(J33202:2007),Craig Venus,T9002,F2620
-:失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008)
+<table width="200" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample No.</td>
+<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td>
+</tr>
+<tr>
+<td>Sample DNA</td>
+<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+</tr>
+<tr>
+<td>loading dye(&mu;L)</td>
+<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+</tr>
+<tr>
+<td>dH<sub>2</sub>O(&mu;L)</td>
+<td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
+</tr>
+<tr>
+<td>Total(&mu;L)</td>
+<td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
+</tr>
+</table>
+-->1,2,3,4,5:none
+'''[[Team:Chiba/protocol/transformation|Transformation]]'''
+*[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
+*[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
+*[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
+*[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
+*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
+*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)-->only one colony
+===Team:Communication===
+:(18/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
+::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
+::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
+::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
+:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+{|align="justify"
+|[[Image:Chiba-0820-3.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample DNA</td>
+<td>3</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>2</td>
+</tr>
+<tr>
+<td>dH<sub>2</sub>O</td>
+<td>7</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>12μL</td>
+</tr>
+</table>
+:From left;
+::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> None
+|}
+{|align="justify"
+|[[Image:Chiba-0820-4.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample DNA</td>
+<td>10</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>4</td>
+</tr>
+<tr>
+<td>dH<sub>2</sub>O</td>
+<td>10</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>24μL</td>
+</tr>
+</table>
+:From left;
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> OK
+::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
+::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007) -> OK
+::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006) -> OK
+|}
+{|align="justify"
+|[[Image:Chiba-0820.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample DNA</td>
+<td>3</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>2</td>
+</tr>
+<tr>
+<td>dH<sub>2</sub>O</td>
+<td>7</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>12μL</td>
+</tr>
+</table>
+:From left;<br>
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
+|}
+--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
+<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample No</td>
+<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td><td>7</td><td>8</td>
+</tr>
+<tr>
+<td>[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)</td>
+<td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td>
+</tr>
+<tr>
+<td>[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)</td>
+<td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td>
+</tr>
+<tr>
+<td>[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)</td>
+<td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td>
+</tr>
+<tr>
+<td>[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)</td>
+<td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td>
+</tr>
+<tr>
+<td>EcoRI</td>
+<td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
+</tr>
+<tr>
+<td>PstI</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
+</tr>
+<tr>
+<td>Buffer EcoRI</td>
+<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+</tr>
+<tr>
+<td>BSA</td>
+<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+</tr>
+<tr>
+<td>dH<sub>2</sub>O</td>
+<td>4.9</td><td>4.9</td><td>4.9</td><td>4.9</td><td>4.8</td><td>4.8</td><td>4.8</td><td>4.8</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td>
+</tr>
+</table>
+:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+{|align="justify"
+|[[Image:Chiba-0820-2.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample DNA</td>
+<td>3</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>2</td>
+</tr>
+<tr>
+<td>dH<sub>2</sub>O</td>
+<td>7</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>12μL</td>
+</tr>
+</table>
+:From left;
+:(Single Digestion 1~4)
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
+:(Double Digestion 1~4)
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
+|}
+===Team:Output===
+[[Team:Chiba/protocol/transformation|Transformation]]
+*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2007)-->colony
+*[http://partsregistry.org/Part:BBa_I712019 BBa_I712019](2008)-->no colony
+*[http://partsregistry.org/Part:BBa_J33202 BBa_J33202](2007)-->colony
+*[http://partsregistry.org/Part:BBa_E2030 BBa_E2030](2007)-->not so good
+*YFP(P-GEX)-->colony(Amp)
+*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->colony
+*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)-->colony

Team:Chiba/Calendar-Home/20 August 2008

From 2008.igem.org

Latest revision as of 05:57, 30 October 2008

Contents

Laboratory work

Team:Input

Team:Communication

Team:Output