Team:Chiba/Calendar-Home/20 August 2008

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(Difference between revisions)

Latest revision as of 05:57, 30 October 2008

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19 August 2008 <|> 21 August 2008

Laboratory work

Team:Input

PCR

[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)(1)
[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)(2)
[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)(3)
[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)(4)
[http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)(5)

Sample No.	1	2	3	4	5
DNA tamplate(μL)	1	1	1	1	1
FW primer(μL)	5	5	5	5	5
VR primer(μL)	5	5	5	5	5
dNTPmix(μL)	10	10	10	10	10
thermo pol buffer(μL)	10	10	10	10	10
DNA pol(VENT)(μL)	1	1	1	1	1
dH₂O(μL)	68	68	68	68	68
TOTAL(μL)	100	100	100	100	100

-->Gel Check

Sample No.	1	2	3	4	5
Sample DNA	1	1	1	1	1
loading dye(μL)	1	1	1	1	1
dH₂O(μL)	4	4	4	4	4
Total(μL)	6	6	6	6	6

-->1,2,3,4,5:none

Transformation

[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)-->only one colony

Team:Communication

(18/8)--->Mini prep

[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)

--->Gel Check

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> None

Sample DNA	10
Loading Dye	4
dH₂O	10
TOTAL	24μL

From left;

[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> OK
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007) -> OK
[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006) -> OK

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK

--->Digestion test

[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)

Sample No	1	2	3	4	5	6	7	8
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)	3	-	-	-	3	-	-	-
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)	-	3	-	-	-	3	-	-
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)	-	-	3	-	-	-	3	-
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)	-	-	-	3	-	-	-	3
EcoRI	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1
PstI	-	-	-	-	0.1	0.1	0.1	0.1
Buffer EcoRI	1	1	1	1	1	1	1	1
BSA	1	1	1	1	1	1	1	1
dH₂O	4.9	4.9	4.9	4.9	4.8	4.8	4.8	4.8
TOTAL	10μL	10μL	10μL	10μL	10μL	10μL	10μL	10μL

--->Gel Check

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

(Single Digestion 1~4)

[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK

(Double Digestion 1~4)

[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK

Team:Output

Transformation

[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2007)-->colony
[http://partsregistry.org/Part:BBa_I712019 BBa_I712019](2008)-->no colony
[http://partsregistry.org/Part:BBa_J33202 BBa_J33202](2007)-->colony
[http://partsregistry.org/Part:BBa_E2030 BBa_E2030](2007)-->not so good
YFP(P-GEX)-->colony(Amp)
[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->colony
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)-->colony

@@ Line 6: / Line 6: @@
 ===Team:Input===
 '''[[Team:Chiba/protocol/PCR|PCR]]'''
-*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)①
+*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)(1)
-*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)②
+*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)(2)
-*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)③
+*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)(3)
-*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)④
+*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)(4)
-*[http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)⑤
+*[http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)(5)
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
 <td width="257">Sample No.</td>
-<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
+<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td>
 </tr>
 <tr>
-<td>DNA tamplate</td>
+<td>DNA tamplate(&mu;L)</td>
-<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
+<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 </tr>
 <tr>
-<td>FW primer</td>
+<td>FW primer(&mu;L)</td>
 <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
 </tr>
 <tr>
-<td>VR primer</td>
+<td>VR primer(&mu;L)</td>
 <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
 </tr>
 <tr>
-<td>dNTPmix</td>
+<td>dNTPmix(&mu;L)</td>
 <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
 </tr>
 <tr>
-<td>thermo pol buffer</td>
+<td>thermo pol buffer(&mu;L)</td>
 <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
 </tr>
 <tr>
-<td>DNA pol(VENT)</td>
+<td>DNA pol(VENT)(&mu;L)</td>
 <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
 <td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
 </tr>
@@ Line 56: / Line 56: @@
 <tr>
 <td width="257">Sample No.</td>
-<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
+<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td>
 </tr>
 <tr>
 <td>Sample DNA</td>
-<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
+<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 </tr>
 <tr>
-<td>loading dye</td>
+<td>loading dye(&mu;L)</td>
 <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
 </tr>
 <tr>
-<td>Total</td>
+<td>Total(&mu;L)</td>
 <td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
 </tr>
@@ Line 77: / Line 77: @@
 </table>
--->どれもバンド出ず。
+-->1,2,3,4,5:none
 '''[[Team:Chiba/protocol/transformation|Transformation]]'''
@@ Line 86: / Line 86: @@
 *[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
 *[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
-*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)
+*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)-->only one colony
--->BBa_J22141のみコロニーがほとんど生えていなかった。(１個のみ)
 ===Team:Communication===
 :(18/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
-::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
+::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
-::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
+::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
-::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
+::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
-::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
-::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
-::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
-::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
-::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
-::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
@@ Line 119: / Line 115: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>7</td>
 </tr>
@@ Line 129: / Line 125: @@
 :From left;
-::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
+::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
-::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
-::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> None
 |}
@@ Line 149: / Line 145: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>10</td>
 </tr>
@@ Line 159: / Line 155: @@
 :From left;
-::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
-::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> OK
+::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> OK
-::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
+::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
-::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
+::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007) -> OK
-::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK
+::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006) -> OK
 |}
@@ Line 182: / Line 178: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>7</td>
 </tr>
@@ Line 192: / Line 188: @@
 :From left;<br>
-::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK<br>
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
-::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK<br>
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
-::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK<br>
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
-::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK<br>
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
 |}
 --->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
-::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
-::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
-::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
-::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
 <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
@@ Line 211: / Line 207: @@
 </tr>
 <tr>
-<td>BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]</td>
+<td>[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)</td>
 <td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td>
 </tr>
 <tr>
-<td>BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]</td>
+<td>[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)</td>
 <td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td>
 </tr>
 <tr>
-<td>BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]</td>
+<td>[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)</td>
 <td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td>
 </tr>
 <tr>
-<td>BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]</td>
+<td>[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)</td>
 <td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td>
 </tr>
 <tr>
-<td>EcoRⅠ</td>
+<td>EcoRI</td>
 <td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstI</td>
 <td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
 </tr>
 <tr>
-<td>Buffer EcoRⅠ</td>
+<td>Buffer EcoRI</td>
 <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 </tr>
@@ Line 243: / Line 239: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>4.9</td><td>4.9</td><td>4.9</td><td>4.9</td><td>4.8</td><td>4.8</td><td>4.8</td><td>4.8</td>
 </tr>
 <tr>
 <td>TOTAL</td>
-<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+<td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td>
 </tr>
 </table>
@@ Line 268: / Line 264: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>7</td>
 </tr>
@@ Line 278: / Line 274: @@
 :From left;
 :(Single Digestion 1~4)
-::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
-::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
-::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
-::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
 :(Double Digestion 1~4)
-::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
-::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
+::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
-::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
-::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
+::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
 |}
 ===Team:Output===
 [[Team:Chiba/protocol/transformation|Transformation]]
-*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](Luciferase Renilla)-->colony
+*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2007)-->colony
-*[http://partsregistry.org/Part:BBa_I712019 BBa_I712019](Luciferase Fire fly)-->no colony
+*[http://partsregistry.org/Part:BBa_I712019 BBa_I712019](2008)-->no colony
-*[http://partsregistry.org/Part:BBa_J33202 BBa_J33202](LacZ)-->colony
+*[http://partsregistry.org/Part:BBa_J33202 BBa_J33202](2007)-->colony
-*[http://partsregistry.org/Part:BBa_E2030 BBa_E2030](Venus YFP)-->not so good
+*[http://partsregistry.org/Part:BBa_E2030 BBa_E2030](2007)-->not so good
-*YFP(P-GEX)-->colony
+*YFP(P-GEX)-->colony(Amp)
-*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]-->colony
+*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->colony
-*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]-->colony
+*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)-->colony
-<br>Luciferase(J52008:2007,2008 I712019:2008)(Amp)
-<br>Lac Z(J33202:2007,2008)(Amp)
-<br>Venus(E2030:2007,2008)(Kam)
-<br>Craig Venus(Amp?Kan?)
-<br>T9002(Amp)
-<br>F2620(Amp)
-<br>luciferase,LacZ,Venus
-<br>competent cell  50μl
-<br>DNA   1μl
-<br>soc  200μl
-<br>plating 250μl
-<br>Craig Venus,T9002,F2620
-<br>competent cell  30μl
-<br>DNA  1μl
-<br>soc  120μl
-<br>plating 20μl
-<br>result
-:成功→Luciferase(J52008:2007),Lac Z(J33202:2007),Craig Venus,T9002,F2620
-:失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008)

Team:Chiba/Calendar-Home/20 August 2008

From 2008.igem.org

Latest revision as of 05:57, 30 October 2008

Contents

Laboratory work

Team:Input

Team:Communication

Team:Output