Team:Hawaii/Meeting/2008-06-30

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(updated with comment from GK)
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## Template DNA: [http://partsregistry.org/wiki/index.php/Part:pSB1A3 pSB1A3]? to PCR out BioBrick Site from pSB1A3 (straight replacement of pRL1383a Multiple Cloning Site -> BioBrick Site)
## Template DNA: [http://partsregistry.org/wiki/index.php/Part:pSB1A3 pSB1A3]? to PCR out BioBrick Site from pSB1A3 (straight replacement of pRL1383a Multiple Cloning Site -> BioBrick Site)
##*GK: Actually, it's not pSB1A3 (RE site problems). The primers will work with any Biobrick plasmid, like pSB1A2 (no RE problems) or pSB2K3. Neither have the ccdB gene and are available on plates 1003/4A and 1014/1F, respectively.
##*GK: Actually, it's not pSB1A3 (RE site problems). The primers will work with any Biobrick plasmid, like pSB1A2 (no RE problems) or pSB2K3. Neither have the ccdB gene and are available on plates 1003/4A and 1014/1F, respectively.
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## Template DNA: Anabaena cells? to PCR out Nir promoter
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## <strike>Template DNA: Anabaena cells? to PCR out Nir promoter</strike> assemble from oligos
# BioBrick Extracted Roundup
# BioBrick Extracted Roundup
## summarize what we extracted and update their status ([http://packrat.stjohn.hawaii.edu/prestinglab/wiki/iGEM:2008/BioBrick_Extraction here], and relocate it to the public Wiki)
## summarize what we extracted and update their status ([http://packrat.stjohn.hawaii.edu/prestinglab/wiki/iGEM:2008/BioBrick_Extraction here], and relocate it to the public Wiki)
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== Action Items ==
== Action Items ==
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* &lt;Person A&gt;: Clean up seed PCC6803 seed stock area (many of them are turning yellow and needs to be thrown away)
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* Margaret
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:* Update project part A description on team wiki (by 7/1)
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*Krystle
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:* Update project part B background/proposal on team wiki (by 7/1)
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:* Update Biobrick extraction list on private website with status of plasmid preps and cryostocks
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* Grace
 +
:* Update project part B methods on team wiki (by 7/1)
 +
* Norman
 +
:* Review and order new primers ASAP
== Coming Up ==
== Coming Up ==

Latest revision as of 00:47, 1 July 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Contents

Agenda

  1. PCR Week! (our 38 primers arrived last Friday.) What else do we need?
    1. Template DNA: [http://partsregistry.org/wiki/index.php/Part:pSB1A3 pSB1A3]? to PCR out BioBrick Site from pSB1A3 (straight replacement of pRL1383a Multiple Cloning Site -> BioBrick Site)
      • GK: Actually, it's not pSB1A3 (RE site problems). The primers will work with any Biobrick plasmid, like pSB1A2 (no RE problems) or pSB2K3. Neither have the ccdB gene and are available on plates 1003/4A and 1014/1F, respectively.
    2. Template DNA: Anabaena cells? to PCR out Nir promoter assemble from oligos
  2. BioBrick Extracted Roundup
    1. summarize what we extracted and update their status ([http://packrat.stjohn.hawaii.edu/prestinglab/wiki/iGEM:2008/BioBrick_Extraction here], and relocate it to the public Wiki)
      • cryostocked
      • plasmid prepped
      • sequence verified
  3. Revisit Project Description (due Tuesday 7/1)
  4. Inventory Walk-through (4C Fridge organization, -20C Freezer shelf organization, -80C Freezer shelf organization)
  5. 2nd Round of Primer Ordering (review and finalize list)

Minutes

Present: <Person A>, <Person B>

Action Items

  • Margaret
  • Update project part A description on team wiki (by 7/1)
  • Krystle
  • Update project part B background/proposal on team wiki (by 7/1)
  • Update Biobrick extraction list on private website with status of plasmid preps and cryostocks
  • Grace
  • Update project part B methods on team wiki (by 7/1)
  • Norman
  • Review and order new primers ASAP

Coming Up

  • 7/1 Project Description Due


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