Team:LCG-UNAM-Mexico/Parts

From 2008.igem.org

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           <td width="165" bgcolor="#5C743D"><a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Team" class="navText">About Us</a></td>
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           <td width="165" bgcolor="#5C743D"><a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Project" class="navText">Our project</a></td>
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           <td width="165" bgcolor="#5C743D"><a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Project" class="navText">Our Project</a></td>
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           <td width="165" bgcolor="#5C743D"><a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Modeling" class="navText">Modeling</a></td>
         </tr>
         </tr>
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           <td width="165" bgcolor="#5C743D"><a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments" class="navText">Experiments</a></td>
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           <td width="165" bgcolor="#5C743D"><a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments" class="navText">Wet Lab</a></td>
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         </tr>
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           <td width="165" bgcolor="#5C743D"><a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Modeling" class="navText">Modeling</a></td>
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           <td width="165" bgcolor="#5C743D"><a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Notebook" class="navText">Notebook</a></td>
         </tr>
         </tr>
         <tr>
         <tr>
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           <td width="165" bgcolor="#5C743D"><a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Notebook" class="navText">Notebook</a></td>
+
           <td width="165" bgcolor="#5C743D"><a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Story" class="navText">Our story</a></td>
 +
        </tr>
 +
<tr>
 +
          <td width="165" bgcolor="#5C743D"><a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Team" class="navText">About us</a></td>
         </tr>
         </tr>
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      </table>
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-
<br />
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    </table> <br />
   &nbsp;<br />
   &nbsp;<br />
   &nbsp;<br />
   &nbsp;<br />
   &nbsp;<br /> </td>
   &nbsp;<br /> </td>
     <td width="42">&nbsp;</td>
     <td width="42">&nbsp;</td>
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     <td colspan="4" valign="top"><img src="https://static.igem.org/mediawiki/2008/7/70/Head_spacer.gif" alt="" width="305" height="1" border="0" /><br />
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     <td colspan="4" valign="top"><div align="center"><img src="https://static.igem.org/mediawiki/2008/7/70/Head_spacer.gif" alt="" width="305" height="1" border="0" /><br />
 +
        <a href="#regparts"><img src="https://static.igem.org/mediawiki/2008/1/1f/Regparts1.jpg" alt="Regulatory parts" width="190" height="31" border="0"></a>    &nbsp;<a href="#codparts"><img src="https://static.igem.org/mediawiki/2008/2/25/Regparts2.jpg" alt="Coding parts" width="190" height="31" border="0"></a><a href="#primers"><img src="https://static.igem.org/mediawiki/2008/8/8b/Regparts3.jpg" alt="Primers" width="190" height="31" border="0"></a><a href="#devices"><img src="https://static.igem.org/mediawiki/2008/d/d9/Regparts4.jpg" alt="Devices" width="190" height="31" border="0"></a><br>
 +
        <img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" alt="ribbon" width="579" height="9" /><br />
&nbsp;<br />
&nbsp;<br />
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&nbsp;<br />
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        </div>
-
        <table border="0" cellspacing="0" cellpadding="2" width="590"><tr>
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      <table border="0" cellspacing="0" cellpadding="2" width="590">
-
           <td class="pageName"><strong>Parts</strong></td>
+
          <tr>
 +
           <td colspan="4" class="pageName"><div align="center">Parts Submitted to the Registry </div></td>
         </tr>
         </tr>
        
        
-
        <tr>
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<tr>
-
           <td class="bodyText"><p>The following are the bioparts that we are bringing into play for our project:</p>
+
           <td colspan="4" class="pageName"><div align="center"><br>
-
<ul>
+
              <span class="style1">Constructed Parts</span></div></td>
-
  <li>BBa_I729006 --- (AHL reporter and aiia device, pTetR+luxR+pLuxR+GFP+pLacI+aiiA)</li>
+
-
  <li>BBa_C0051 --- (cI repressor from E. coli phage lambda (+LVA))</li>
+
-
  <li>BBa_R0051 --- (promoter (lambda cI regulated))</li>
+
-
  </ul>
+
-
</td>
+
-
</tr>
+
-
<tr>
+
-
<tr>
+
-
          <td class="pageName">Constructed Parts</td>
+
         </tr>
         </tr>
<tr>
<tr>
-
          <td class="quote">...Comming soon...</td>
+
  <td colspan="4" class="bodyText">The following are the bioparts that we designed for our project:</td>
-
          </tr>
+
</tr>
-
    </table><br />
+
<tr>
-
&nbsp;<br />
+
  <td colspan="3" class="calHeader">&nbsp;</td>
 +
  <td class="calHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="3" class="calHeader style2"><a name="regparts"></a>Regulatory Parts</td>
 +
  <td width="337" class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png" align="left
 +
  "></td>
 +
  </tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">BioBrick Number:</td>
 +
  <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119000">BBa_K119000</a></h1></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Part Name:</td>
 +
  <td colspan="2" class="bodyText"><strong>Constitutive weak promoter of lacZ</strong></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Description:</td>
 +
  <td colspan="2" class="bodyText">LacZ constitutive promoter, without operators. </td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Length:</td>
 +
  <td colspan="2" class="bodyText">38 bp</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Designed by:</td>
 +
  <td colspan="2" class="bodyText">Miguel Angel Ramirez-Romero</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">References:</td>
 +
  <td colspan="2" class="bodyText">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">&nbsp;</td>
 +
  <td colspan="2" class="calHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">BioBrick Number:</td>
 +
  <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119001">BBa_K119001</a></h1></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Part Name:</td>
 +
  <td colspan="2" class="bodyText"><strong>Mutated LacZ promoter</strong></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >Description:</td>
 +
  <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Constitutive weak promoter of lacZ which sequence was changed at position 29 (T-&gt;G) in order to make it weaker</div></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" align="up">&nbsp;</td>
 +
  </tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Length:</td>
 +
  <td colspan="2" class="bodyText">38 bp</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Designed by:</td>
 +
  <td colspan="2" class="bodyText">Miguel Angel Ramirez-Romero</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">References:</td>
 +
  <td colspan="2" class="bodyText">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">&nbsp;</td>
 +
  <td colspan="2" class="calHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">BioBrick Number:</td>
 +
  <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119002">BBa_K119002</a></h1></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Part Name:</td>
 +
  <td colspan="2" class="bodyText"><strong>RcnR operator (represses RcnA)</strong></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >Description:</td>
 +
  <td colspan="2" class="bodyText">Operator for RcnR(YohL) the repressor of nickel extrusion pump(RcnA) in <i>E.coli</i> </td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Length:</td>
 +
  <td colspan="2" class="bodyText">83 bp</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Designed by:</td>
 +
  <td colspan="2" class="bodyText">Libertad Pantoja</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">References:</td>
 +
  <td colspan="2" class="bodyText">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">&nbsp;</td>
 +
  <td colspan="2" class="calHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="3" class="calHeader style2"><a name="codparts"></a>Coding Parts</td>
 +
  <td class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_coding.png" alt="" align="left
 +
  "></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">BioBrick Number:</td>
 +
  <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119003">BBa_K119003</a></h1></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Part Name:</td>
 +
  <td colspan="2" class="bodyText"><strong>RcnA (YohM)</strong></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >Description:</td>
 +
  <td colspan="2" class="bodyText">Nickel efflux pump from <i>E.coli</i></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Length:</td>
 +
  <td colspan="2" class="bodyText">833 bp</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Designed by:</td>
 +
  <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad  Pantoja, B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur  Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">&nbsp;</td>
 +
  </tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">References:</td>
 +
  <td colspan="2" class="bodyText">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">&nbsp;</td>
 +
  <td colspan="2" class="calHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="3" class="calHeader style2"><a name="primers"></a>Primers</td>
 +
  <td class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_primer.png" alt="" align="left
 +
  "></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">BioBrick Number:</td>
 +
  <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119004">BBa_K119004</a></h1></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Part Name:</td>
 +
  <td colspan="2" class="bodyText"><strong>Upper primer for <em>aiiA</em> with normal lacZ promoter </strong></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >Description:</td>
 +
  <td colspan="2" rowspan="2" class="bodyText"><p align="justify">Upper primer used to add lacZ promoter  to  aiiA in the device  LuxR_cI_aiiA_Normal lacZ promoter </p></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >&nbsp;</td>
 +
  </tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Length:</td>
 +
  <td colspan="2" class="bodyText">67 bp</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Designed by:</td>
 +
  <td colspan="2" class="bodyText">Martin del Castillo V.</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">References:</td>
 +
  <td colspan="2" class="bodyText">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">&nbsp;</td>
 +
  <td colspan="2" class="calHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">BioBrick Number:</td>
 +
  <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119005">BBa_K119005</a></h1></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Part Name:</td>
 +
  <td colspan="2" class="bodyText"><strong>Upper primer for <em>aiiA</em> with mutated lacZ promoter  </strong></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >Description:</td>
 +
  <td colspan="2" rowspan="2" class="bodyText"><p align="justify">Upper primer used to add a mutated version of lacZ promoter  to  <em>aiiA</em> in the device  LuxR_cI_aiiA_Normal lacZ promoter  </p></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Length:</td>
 +
  <td colspan="2" class="bodyText">67 bp</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Designed by:</td>
 +
  <td colspan="2" class="bodyText">Martin del Castillo V.</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">References:</td>
 +
  <td colspan="2" class="bodyText">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">&nbsp;</td>
 +
  <td colspan="2" class="calHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">BioBrick Number:</td>
 +
  <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119007">BBa_K119007</a></h1></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Part Name:</td>
 +
  <td colspan="2" class="bodyText"><strong>Upper primer for RcnA  </strong></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >Description:</td>
 +
  <td colspan="2" rowspan="2" class="bodyText"><p align="justify">Upper primer used to add cI promoter upstream of the RcnR operator in the device cI promoter RcnR op RcnA </p></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Length:</td>
 +
  <td colspan="2" class="bodyText">73 bp</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Designed by:</td>
 +
  <td colspan="2" class="bodyText">Libertad Pantoja</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">References:</td>
 +
  <td colspan="2" class="bodyText">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">&nbsp;</td>
 +
  <td colspan="2" class="calHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">BioBrick Number:</td>
 +
  <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119008">BBa_K119008</a></h1></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Part Name:</td>
 +
  <td colspan="2" class="bodyText"><strong>Lower primer for RcnA (BBa_K119003) </strong></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >Description:</td>
 +
  <td colspan="2" class="bodyText"><p align="justify">Lower primer for RcnA (BBa_K119003). Includes only the end of the RcnA coding region.</p></td>
 +
</tr>
 +
 
 +
<tr>
 +
  <td colspan="2" class="subHeader">Length:</td>
 +
  <td colspan="2" class="bodyText">21 bp</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Designed by:</td>
 +
  <td colspan="2" class="bodyText">Libertad Pantoja</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">References:</td>
 +
  <td colspan="2" class="bodyText">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">&nbsp;</td>
 +
  <td colspan="2" class="calHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="3" class="calHeader style2"><a name="devices"></a>Devices</td>
 +
  <td class="calHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">BioBrick Number:</td>
 +
  <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119009">BBa_K119009</a></h1></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Part Name:</td>
 +
  <td colspan="2" class="bodyText"><strong>cI promoter RcnR op RcnA </strong></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >Description:</td>
 +
  <td colspan="2" rowspan="2" class="bodyText"><p align="justify">This device encodes the extrusion pump and uses two repressible  promoters in order to stop the niquel extrusion. </p></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >&nbsp;</td>
 +
  </tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Length:</td>
 +
  <td colspan="2" class="bodyText">969 bp</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Designed by:</td>
 +
  <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad  Pantoja, B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur  Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">References:</td>
 +
  <td colspan="2" class="bodyText">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">&nbsp;</td>
 +
  <td colspan="2" class="calHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">BioBrick Number:</td>
 +
  <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119010">BBa_K119010</a></h1></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Part Name:</td>
 +
  <td colspan="2" class="bodyText"><strong>LuxR_cI_aiiA_Normal lacZ promoter  </strong></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >Description:</td>
 +
  <td colspan="2" rowspan="2" class="bodyText"><p align="justify">This device encodes CI, the protein which controls <em>rcnA</em> expression. It also contains <em>luxR</em>, which induces the expression of <em>cI</em> and <em>aiiA.</em></p></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Length:</td>
 +
  <td colspan="2" class="bodyText">2873 bp</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Designed by:</td>
 +
  <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">References:</td>
 +
  <td colspan="2" class="bodyText">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">&nbsp;</td>
 +
  <td colspan="2" class="calHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">BioBrick Number:</td>
 +
  <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119011">BBa_K119011</a></h1></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Part Name:</td>
 +
  <td colspan="2" class="bodyText"><strong>LuxR_cI_aiiA_Mutated lacZ promoter  </strong></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >Description:</td>
 +
  <td colspan="2" rowspan="2" class="bodyText"><p align="justify">This device encodes CI, the protein which controls <em>rcnA</em>. It also contains <em>luxR</em>, which induces the expression of <em>cI</em> and <em>aiiA</em> </p></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader" >&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Length:</td>
 +
  <td colspan="2" class="bodyText">2873 bp</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">Designed by:</td>
 +
  <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="subHeader">References:</td>
 +
  <td colspan="2" class="bodyText">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td colspan="2" class="calHeader">&nbsp;</td>
 +
  <td colspan="2" class="calHeader">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td width="108" class="calHeader">&nbsp;</td>
 +
  <td width="33" class="calHeader">&nbsp;</td>
 +
  <td colspan="2" class="calHeader">&nbsp;</td>
 +
  </tr>    </table>
 +
      <div align="center"><br />
 +
        &nbsp;<a href="#regparts"><img src="https://static.igem.org/mediawiki/2008/1/1f/Regparts1.jpg" alt="Regulatory parts" width="190" height="31" border="0"></a> &nbsp;<a href="#codparts"><img src="https://static.igem.org/mediawiki/2008/2/25/Regparts2.jpg" alt="Coding parts" width="190" height="31" border="0"></a><a href="#primers"><img src="https://static.igem.org/mediawiki/2008/8/8b/Regparts3.jpg" alt="Primers" width="190" height="31" border="0"></a><a href="#devices"><img src="https://static.igem.org/mediawiki/2008/d/d9/Regparts4.jpg" alt="Devices" width="190" height="31" border="0"></a><br>
 +
        <br>
 +
        <img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" alt="ribbon" width="579" height="9" /><br />
 +
  &nbsp;<br />
 +
  <br />
 +
        </div>
     <td width="132">&nbsp;</td>
     <td width="132">&nbsp;</td>
   </tr>
   </tr>

Latest revision as of 01:34, 26 November 2008

LCG-UNAM-Mexico:Parts

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iGEM 2008 TEAM
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Regulatory parts  Coding partsPrimersDevices
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Parts Submitted to the Registry

Constructed Parts
The following are the bioparts that we designed for our project:
   
Regulatory Parts
BioBrick Number:

BBa_K119000
Part Name: Constitutive weak promoter of lacZ
Description: LacZ constitutive promoter, without operators.
Length: 38 bp
Designed by: Miguel Angel Ramirez-Romero
References:  
   
BioBrick Number:

BBa_K119001
Part Name: Mutated LacZ promoter
Description:
Constitutive weak promoter of lacZ which sequence was changed at position 29 (T->G) in order to make it weaker
 
Length: 38 bp
Designed by: Miguel Angel Ramirez-Romero
References:  
   
BioBrick Number:

BBa_K119002
Part Name: RcnR operator (represses RcnA)
Description: Operator for RcnR(YohL) the repressor of nickel extrusion pump(RcnA) in E.coli
Length: 83 bp
Designed by: Libertad Pantoja
References:  
   
Coding Parts
BioBrick Number:

BBa_K119003
Part Name: RcnA (YohM)
Description: Nickel efflux pump from E.coli
Length: 833 bp
Designed by:
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo
 
References:  
   
Primers
BioBrick Number:

BBa_K119004
Part Name: Upper primer for aiiA with normal lacZ promoter
Description:

Upper primer used to add lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter

 
Length: 67 bp
Designed by: Martin del Castillo V.
References:  
   
BioBrick Number:

BBa_K119005
Part Name: Upper primer for aiiA with mutated lacZ promoter
Description:

Upper primer used to add a mutated version of lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter

 
Length: 67 bp
Designed by: Martin del Castillo V.
References:  
   
BioBrick Number:

BBa_K119007
Part Name: Upper primer for RcnA
Description:

Upper primer used to add cI promoter upstream of the RcnR operator in the device cI promoter RcnR op RcnA

 
Length: 73 bp
Designed by: Libertad Pantoja
References:  
   
BioBrick Number:

BBa_K119008
Part Name: Lower primer for RcnA (BBa_K119003)
Description:

Lower primer for RcnA (BBa_K119003). Includes only the end of the RcnA coding region.
Length: 21 bp
Designed by: Libertad Pantoja
References:  
   
Devices  
BioBrick Number:

BBa_K119009
Part Name: cI promoter RcnR op RcnA
Description:

This device encodes the extrusion pump and uses two repressible promoters in order to stop the niquel extrusion.

 
Length: 969 bp
Designed by:
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo
 
References:  
   
BioBrick Number:

BBa_K119010
Part Name: LuxR_cI_aiiA_Normal lacZ promoter
Description:

This device encodes CI, the protein which controls rcnA expression. It also contains luxR, which induces the expression of cI and aiiA.
 
Length: 2873 bp
Designed by:
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo
References:  
   
BioBrick Number:

BBa_K119011
Part Name: LuxR_cI_aiiA_Mutated lacZ promoter
Description:

This device encodes CI, the protein which controls rcnA. It also contains luxR, which induces the expression of cI and aiiA

 
Length: 2873 bp
Designed by:
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo
References:  
   
     

 Regulatory parts  Coding partsPrimersDevices

ribbon
 

 
             

Retrieved from "http://2008.igem.org/Team:LCG-UNAM-Mexico/Parts"