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Team:CityColSanFrancisco/Notebook/LabBooks/Colby
From 2008.igem.org
(New page: '''6/25''' <br> Lab book created. All future work done by me will be posted on this document. <br> Following yesterday's plating of S.'' oneidensis'' plates were observed to search for g...) |
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'''6/25''' <br> | '''6/25''' <br> | ||
Lab book created. All future work done by me will be posted on this document. <br> | Lab book created. All future work done by me will be posted on this document. <br> | ||
| - | Following yesterday's plating of S.'' oneidensis'' plates were observed to search for growth. No growth was detected. This is rather disturbing as oneidensis is supposed to be our fastest grower. What went wrong? Perhaps our media is bad or maybe our incubator is running too hot/cold. | + | Following yesterday's plating of S.'' oneidensis'' plates were observed to search for growth. No growth was detected. This is rather disturbing as oneidensis is supposed to be our fastest grower. What went wrong? Perhaps our media is bad or maybe our incubator is running too hot/cold. <br> |
| + | We will test our media first. New media was made using same procedure as previous batch (the only difference being that 20g of agar was used instead of 15g). Plates were poured using this new batch. We will determine if our LB (from the previous batch) is defective by plating 1 plate from our previous LB, 1 from a plate borrowed from the Btech program, and 1 plate from our new batch of LB. Hopefully there will be some growth by tomorrow on at least one plate. <br> | ||
Revision as of 16:40, 26 June 2009
6/25
Lab book created. All future work done by me will be posted on this document.
Following yesterday's plating of S. oneidensis plates were observed to search for growth. No growth was detected. This is rather disturbing as oneidensis is supposed to be our fastest grower. What went wrong? Perhaps our media is bad or maybe our incubator is running too hot/cold.
We will test our media first. New media was made using same procedure as previous batch (the only difference being that 20g of agar was used instead of 15g). Plates were poured using this new batch. We will determine if our LB (from the previous batch) is defective by plating 1 plate from our previous LB, 1 from a plate borrowed from the Btech program, and 1 plate from our new batch of LB. Hopefully there will be some growth by tomorrow on at least one plate.
