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Minnesota/26 June 2008
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| + | |'''[[Team:Minnesota/NotebookComparator| Back to Notebook Home]]''' | ||
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| + | |'''[[Minnesota/25 June 2008|Go to Previous Day (June 25)]]'''|| width=158|'''[[Minnesota/27 June 2008|Go to Next Day (June 27)]]''' | ||
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|1. '''Glycerol Stock:''' | |1. '''Glycerol Stock:''' | ||
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| - | |a. Add 900uL of glycerol to empty tube | + | |a. Add 900uL of glycerol to empty tube. |
|- | |- | ||
| - | |b. Add 900uL of cells to the glycerol tubes | + | |b. Add 900uL of cells to the glycerol tubes. |
|- | |- | ||
|c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes. | |c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes. | ||
Latest revision as of 15:55, 1 July 2008
| Back to Notebook Home | |
| Go to Previous Day (June 25) | Go to Next Day (June 27) |
| 1. Glycerol Stock: |
| a. Add 900uL of glycerol to empty tube. |
| b. Add 900uL of cells to the glycerol tubes. |
| c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes. |
| d. Place tubes in freezer @ -79C for future use. |
