Minnesota/26 June 2008

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(New page: {| |- |1. '''Glycerol Stock:''' |- |a. Add 900uL of glycerol to empty tube |- |b. Add 900uL of cells to the glycerol tubes |- |c. Immediately place tubes in a beaker that has ethanol and ...)
 
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|1. '''Glycerol Stock:'''
|1. '''Glycerol Stock:'''
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|a. Add 900uL of glycerol to empty tube
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|a. Add 900uL of glycerol to empty tube.
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|b. Add 900uL of cells to the glycerol tubes
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|b. Add 900uL of cells to the glycerol tubes.
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|c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes.
|c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes.
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|d. Place tubes in freezer @ -79C
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|d. Place tubes in freezer @ -79C for future use.
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Latest revision as of 15:55, 1 July 2008

Back to Notebook Home
Go to Previous Day (June 25)Go to Next Day (June 27)
1. Glycerol Stock:
a. Add 900uL of glycerol to empty tube.
b. Add 900uL of cells to the glycerol tubes.
c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes.
d. Place tubes in freezer @ -79C for future use.