Team:KULeuven/Tools

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(Difference between revisions)

Revision as of 12:42, 3 July 2008

Under Construction...

Current issues:

Templates can't be stored within the Team namespace!
They must either be stored under the Template namespace or the User namespace.

A mail has been sent to Meagan of the IGEM team to see whether it is allowed to have a team folder under the 
Template namespace.

Templates

General

Navigation Bar

Usage: {{:Team:KULeuven/Tools/Navigation_Bar}}

Lab Templates

PCR sheet

Usage:

 {{:Team:KULeuven/Tools/PCR
 |tanneal=55
 |telong=2'30"
 |ncycles=30
 |buffer=5x 10μL
 |mgcl=10μM 0μL
 |dntp=10μM 1μL
 |fwprimername=6 LacZ-Fw
 |fwprimerconc=10μM 2.5μL
 |rvprimername=7 LacZ-Rv
 |rvprimerconc=10μM 2.5μL
 |water=34μL
 |polymerase=Taq 0.5μL
 |dna=0.5μL of E. coli DNA extract
 |size=952bp
 |success=YES/NO
 |image=pcrproducts_GE.jpg
 |lane=3+4
 }}

Result:

PCR:

PCR Settings

Annealing (°C)

55°

Time Elongation

2'30"

Number of Cycles

30

Buffer (5x)	5x 10μL
MgCl₂ 10 µM	10μM 0μL
dNTP 10 µM	10μM 1μL
Oligo F 10 µM	6 LacZ-Fw	10μM 2.5μL
Oligo R 10 µM	7 LacZ-Rv	10μM 2.5μL
Water	34μL
Polymerase	Taq 0.5μL
DNA	0.5μL of E. coli DNA extract

Expected Size

952bp

Success

YES/NO

Image (click to enlarge)

30px

Lane (0=ladder)

3+4

Digestion sheet

Usage: ...

Ligation sheet

Usage: ...

CSS

Calendar CSS file required for notebook.

@@ Line 16: / Line 16: @@
 ==== [[Team:KULeuven/Tools/PCR | PCR sheet]] ====
-'''Usage'''
+'''Usage:'''
   <nowiki>
   {{:Team:KULeuven/Tools/PCR
@@ Line 39: / Line 39: @@
   </nowiki>
-'''Result'''
+'''Result:'''
 {{:Team:KULeuven/Tools/PCR