Team:KULeuven/Tools

From 2008.igem.org

(Difference between revisions)
m (→Lab Templates)
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  Template namespace.
  Template namespace.
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==Templates==
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== Templates ==
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===General===
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=== General ===
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[[Team:KULeuven/Tools/Navigation_Bar | Navigation Bar]]
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==== [[Team:KULeuven/Tools/Navigation_Bar | Navigation Bar]] ====
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Usage: <nowiki>{{:Team:KULeuven/Tools/Navigation_Bar}}</nowiki>
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===Lab Templates===
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'''Usage:'''
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<nowiki>
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{{:Team:KULeuven/Tools/Navigation_Bar}}
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</nowiki>
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=== Lab Templates ===
==== [[Team:KULeuven/Tools/PCR | PCR sheet]] ====
==== [[Team:KULeuven/Tools/PCR | PCR sheet]] ====
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}}
}}
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[[Team:KULeuven/Tools/Digestion | Digestion sheet]]
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==== [[Team:KULeuven/Tools/Digestion | Digestion sheet]] ====
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Usage: ...
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[[Team:KULeuven/Tools/Ligation | Ligation sheet]]
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'''Usage:'''
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Usage: ...
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==== [[Team:KULeuven/Tools/Ligation | Ligation sheet]] ====
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'''Usage:'''
==CSS==
==CSS==
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[[User:Avdmeers/SimpleCalendar.css | Calendar CSS file]] required for notebook.
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==== [[User:Avdmeers/SimpleCalendar.css | Calendar CSS file]] ====

Revision as of 13:40, 3 July 2008

Under Construction... 

Current issues:

Templates can't be stored within the Team namespace!
They must either be stored under the Template namespace or the User namespace.

A mail has been sent to Meagan of the IGEM team to see whether it is allowed to have a team folder under the 
Template namespace.

Contents

Templates

General

Navigation Bar

Usage: 

 {{:Team:KULeuven/Tools/Navigation_Bar}}

Lab Templates

PCR sheet

Usage: 

 {{:Team:KULeuven/Tools/PCR
 |tanneal=55
 |telong=2'30"
 |ncycles=30
 |buffer=5x 10μL
 |mgcl=10μM 0μL
 |dntp=10μM 1μL
 |fwprimername=6 LacZ-Fw
 |fwprimerconc=10μM 2.5μL
 |rvprimername=7 LacZ-Rv
 |rvprimerconc=10μM 2.5μL
 |water=34μL
 |polymerase=Taq 0.5μL
 |dna=0.5μL of E. coli DNA extract
 |size=952bp
 |success=YES/NO
 |image=pcrproducts_GE.jpg
 |lane=3+4
 }}

Result:

PCR:
PCR Settings
Annealing (°C)
55°
Time Elongation
2'30"
Number of Cycles
30
Buffer (5x) 5x 10μL
MgCl2 10 µM 10μM 0μL
dNTP 10 µM 10μM 1μL
Oligo F 10 µM 6 LacZ-Fw 10μM 2.5μL
Oligo R 10 µM 7 LacZ-Rv 10μM 2.5μL
Water 34μL
Polymerase Taq 0.5μL
DNA 0.5μL of E. coli DNA extract
Expected Size
952bp
Success
YES/NO
Image (click to enlarge)
30px
Lane (0=ladder)
3+4

Digestion sheet

Usage:

Ligation sheet

Usage:

CSS

Calendar CSS file

Retrieved from "http://2008.igem.org/Team:KULeuven/Tools"