Team:KULeuven/Tools

From 2008.igem.org

(Difference between revisions)
m
Line 1: Line 1:
Under Construction...
Under Construction...
-
 
-
Current issues:
 
-
 
-
Templates can't be stored within the Team namespace!
 
-
They must either be stored under the Template namespace or the User namespace.
 
-
 
-
A mail has been sent to Meagan of the IGEM team to see whether it is allowed to have a team folder under the
 
-
Template namespace.
 
== Templates ==
== Templates ==
Line 14: Line 6:
'''Usage:'''
'''Usage:'''
-
  <nowiki>
+
  <nowiki>{{:Team:KULeuven/Tools/Navigation_Bar}}</nowiki>
-
{{:Team:KULeuven/Tools/Navigation_Bar}}
+
-
</nowiki>
+
=== Lab Templates ===
=== Lab Templates ===
Line 22: Line 12:
'''Usage:'''
'''Usage:'''
-
  <nowiki>
+
  <nowiki>{{:Team:KULeuven/Tools/PCR
-
{{:Team:KULeuven/Tools/PCR
+
  |tanneal=55
  |tanneal=55
  |telong=2'30"
  |telong=2'30"
Line 41: Line 30:
  |image=pcrproducts_GE.jpg
  |image=pcrproducts_GE.jpg
  |lane=3+4
  |lane=3+4
-
  }}
+
  }}</nowiki>
-
</nowiki>
+
'''Result:'''
'''Result:'''

Revision as of 13:42, 3 July 2008

Under Construction...

Contents

Templates

General

Navigation Bar

Usage: 

{{:Team:KULeuven/Tools/Navigation_Bar}}

Lab Templates

PCR sheet

Usage: 

{{:Team:KULeuven/Tools/PCR
 |tanneal=55
 |telong=2'30"
 |ncycles=30
 |buffer=5x 10μL
 |mgcl=10μM 0μL
 |dntp=10μM 1μL
 |fwprimername=6 LacZ-Fw
 |fwprimerconc=10μM 2.5μL
 |rvprimername=7 LacZ-Rv
 |rvprimerconc=10μM 2.5μL
 |water=34μL
 |polymerase=Taq 0.5μL
 |dna=0.5μL of E. coli DNA extract
 |size=952bp
 |success=YES/NO
 |image=pcrproducts_GE.jpg
 |lane=3+4
 }}

Result:

PCR:
PCR Settings
Annealing (°C)
55°
Time Elongation
2'30"
Number of Cycles
30
Buffer (5x) 5x 10μL
MgCl2 10 µM 10μM 0μL
dNTP 10 µM 10μM 1μL
Oligo F 10 µM 6 LacZ-Fw 10μM 2.5μL
Oligo R 10 µM 7 LacZ-Rv 10μM 2.5μL
Water 34μL
Polymerase Taq 0.5μL
DNA 0.5μL of E. coli DNA extract
Expected Size
952bp
Success
YES/NO
Image (click to enlarge)
30px
Lane (0=ladder)
3+4

Digestion sheet

Usage:

Ligation sheet

Usage:

CSS

Calendar CSS file

Retrieved from "http://2008.igem.org/Team:KULeuven/Tools"