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Team:KULeuven/Tools
From 2008.igem.org
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Revision as of 14:06, 3 July 2008
Under Construction...
Contents |
Templates
General
Usage:
{{:Team:KULeuven/Tools/Navigation_Bar}}
Lab Templates
PCR sheet
Usage:
{{:Team:KULeuven/Tools/PCR
|tanneal=55
|telong=2'30"
|ncycles=30
|buffer=5x 10μL
|mgcl=10μM 0μL
|dntp=10μM 1μL
|fwprimername=6 LacZ-Fw
|fwprimerconc=10μM 2.5μL
|rvprimername=7 LacZ-Rv
|rvprimerconc=10μM 2.5μL
|water=34μL
|polymerase=Taq 0.5μL
|dna=0.5μL of E. coli DNA extract
|size=952bp
|success=YES/NO
|image=pcrproducts_GE.jpg
|lane=3+4
}}
Result:
| PCR: | |||||||||||||||||||||||||||||||||||||||||
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Digestion sheet
Usage:
{{:Team:KULeuven/Tools/Digestion
|nr=D12
|product=Cre ORF
|matrix=pJ61047
|enz1=Pst1
|enz2=Xba1
|size=1063bp
|desc=Cre ORF extracted for further usage
}}
Result:
| Digestion Products | ||||||
|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description |
| D12 | Cre ORF | pJ61047 | XbaI | PstI | 1063bp | Cre ORF extracted for further usage |
Ligation sheet
Usage:
{{:Team:KULeuven/Tools/Ligation
|nr=L21
|insert=D12 Cre ORF
|insert_vol=6µL
|vector=D1 pJ23100
|vector_vol=2µL
|comm=construction of BioBrick: Cre-recombinase under control of strong promotor
|ncol=30
}}
Result
| Ligations | ||||||
|---|---|---|---|---|---|---|
| Number | Insert | Insert Volume (µL) | Vector | Vector Volume (µL) | Comments | Number of colonies |
| L21 | D12 Cre ORF | 6µL | D1 pJ23100 | 2µL | construction of BioBrick: Cre-recombinase under control of strong promotor | 30 |
