Team:Hawaii/Notebook/2008-07- 2

From 2008.igem.org

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:* Restreaked colonies on fresh LB+anitbiotic plates
:* Restreaked colonies on fresh LB+anitbiotic plates
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== Large-Scale Plasmid prep of BioBricks ==
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:<strong> Margaret </strong>
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[[Image:biobrick_plasmid_prep_gel.JPG|right|thumb|This is the gel of the plasmid preps, plus some PCR products of NW and MR.]]
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{| align="center" border="1"
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|+ '''Biobrick Plasmid Prep'''
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!width="200"|Lane
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!width="200"|Biobrick/Other
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!width="200"|description
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|-
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|align="center"|1
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|align="center"|tri-dye ladder
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|
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|-
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|align="center"|2
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|align="center"|B0030
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|
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|-
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|align="center"|3
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|align="center"|R0010
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|
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|-
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|align="center"|4
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|align="center"|C0012
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|
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|-
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|align="center"|5
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|align="center"|E0040
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|
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|-
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|align="center"|6
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|align="center"|J33207
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|
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|-
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|align="center"|7
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|align="center"|rep region
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|
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|-
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|align="center"|8
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|align="center"|(+) control
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|
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|-
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|align="center"|9
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|align="center"|(-) control
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|
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|-
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|align="center"|10
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|align="center"|NW
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|
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|}
===Remade seed stocks of cyanbacteria===
===Remade seed stocks of cyanbacteria===

Revision as of 01:06, 4 July 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Checked for Biobrick transformants

Grace
Biobrick transformants
Biobrick Colonies
B0014 none
B0015 4
E0026 4
E0240 none
I14032 none
J04430 none


  • Restreaked colonies on fresh LB+anitbiotic plates

Large-Scale Plasmid prep of BioBricks

Margaret
This is the gel of the plasmid preps, plus some PCR products of NW and MR.
Biobrick Plasmid Prep
Lane Biobrick/Other description
1 tri-dye ladder
2 B0030
3 R0010
4 C0012
5 E0040
6 J33207
7 rep region
8 (+) control
9 (-) control
10 NW

Remade seed stocks of cyanbacteria

Krystle

Drylab Work

Name of Task

name of person/people who performed the task
  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. read through papers, worked on proposal, etc.

Discussion

  • Use commercial-grade competent cells for E0240 and I14032 extraction? We really need these two Biobricks.

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


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