Revision as of 21:55, 7 July 2008

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Things we did today

Grace

Margaret

This is the gel of the plasmid preps, plus some PCR products of NW and MR.

**Biobrick Plasmid Prep**
Lane	Biobrick/Other	description
1	tri-dye ladder	very light, no difference in intensity
2	B0030	1.75kb, brighter than ladder, a lot of RNA
3	R0010	2.1kb, brighter than ladder, No RNA??
4	C0012	2.5kb, brighter than ladder, a lot of RNA
5	E0040	2.4kb, brighter than ladder, a lot of RNA
6	J33207	2.5kb, maybe 5.7ng/uL, No RNA??
7	rep region	0.5kb, 5.7ng/uL
8	(+) control	bright band below 0.5kb
9	(-) control	all clear
10	SigE	~1kb, maybe 5.7ng/uL

Future Work for this experiment

There is salt contamination in the plasmid preps. This is shown in the smeared bands. This occurred because the ethanol did not completely dry. We need to purify.

Krystle

name of person/people who performed the task

Summary of task and what was done. Link to experiment for detailed notes if necessary.
e.g. read through papers, worked on proposal, etc.

Use commercial-grade competent cells for E0240 and I14032 extraction? We really need these two Biobricks.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 32: / Line 32: @@
 :* Restreaked colonies on fresh LB+anitbiotic plates
-== Large-Scale Plasmid prep of BioBricks ==
+=== Large-Scale Plasmid prep of BioBricks ===
 :<strong> Margaret </strong>