Latest revision as of 21:55, 7 July 2008

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Things we did today

Wetlab work

Checked for Biobrick transformants

Grace

**Biobrick transformants**
Biobrick	Colonies
B0014	none
B0015	4
E0026	4
E0240	none
I14032	none
J04430	none

Restreaked colonies on fresh LB+anitbiotic plates

Large-Scale Plasmid prep of BioBricks

Margaret

This is the gel of the plasmid preps, plus some PCR products of NW and MR.

**Biobrick Plasmid Prep**
Lane	Biobrick/Other	description
1	tri-dye ladder	very light, no difference in intensity
2	B0030	1.75kb, brighter than ladder, a lot of RNA
3	R0010	2.1kb, brighter than ladder, No RNA??
4	C0012	2.5kb, brighter than ladder, a lot of RNA
5	E0040	2.4kb, brighter than ladder, a lot of RNA
6	J33207	2.5kb, maybe 5.7ng/uL, No RNA??
7	rep region	0.5kb, 5.7ng/uL
8	(+) control	bright band below 0.5kb
9	(-) control	all clear
10	SigE	~1kb, maybe 5.7ng/uL

Future Work for this experiment

There is salt contamination in the plasmid preps. This is shown in the smeared bands. This occurred because the ethanol did not completely dry. We need to purify.

Remade seed stocks of cyanbacteria

Krystle

Discussion

Use commercial-grade competent cells for E0240 and I14032 extraction? We really need these two Biobricks.

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 8: / Line 8: @@
 {| align="center" border="1"
 |+ '''Biobrick transformants'''
-!Biobrick
+!width="200"|Biobrick
-!Colonies
+!width="200"|Colonies
 |-
 | align="center"|B0014
@@ Line 28: / Line 28: @@
 |align="center"| J04430
 | align="center"|none
-|-|}
+|}
 <br>
+:* Restreaked colonies on fresh LB+anitbiotic plates
+=== Large-Scale Plasmid prep of BioBricks ===
+:<strong> Margaret </strong>
+[[Image:biobrick_plasmid_prep_gel.JPG|right|thumb|This is the gel of the plasmid preps, plus some PCR products of NW and MR.]]
+{| align="center" border="1"
+|+ '''Biobrick Plasmid Prep'''
+!width="200"|Lane
+!width="200"|Biobrick/Other
+!width="200"|description
+|-
+|align="center"|1
+|align="center"|tri-dye ladder
+| very light, no difference in intensity
+|-
+|align="center"|2
+|align="center"|B0030
+|1.75kb, brighter than ladder, a lot of RNA
+|-
+|align="center"|3
+|align="center"|R0010
+|2.1kb, brighter than ladder, No RNA??
+|-
+|align="center"|4
+|align="center"|C0012
+|2.5kb, brighter than ladder, a lot of RNA
+|-
+|align="center"|5
+|align="center"|E0040
+|2.4kb, brighter than ladder, a lot of RNA
+|-
+|align="center"|6
+|align="center"|J33207
+|2.5kb, maybe 5.7ng/uL, No RNA??
+|-
+|align="center"|7
+|align="center"|rep region
+|0.5kb, 5.7ng/uL
+|-
+|align="center"|8
+|align="center"|(+) control
+|bright band below 0.5kb
+|-
+|align="center"|9
+|align="center"|(-) control
+|all clear
+|-
+|align="center"|10
+|align="center"|SigE
+|~1kb, maybe 5.7ng/uL
+|}
+<strong>Future Work for this experiment</strong>
+*There is salt contamination in the plasmid preps. This is shown in the smeared bands. This occurred because the ethanol did not completely dry. We need to purify.
 ===Remade seed stocks of cyanbacteria===
 :<strong>Krystle</strong>
-== Drylab Work ==
-===Name of Task===
-:<strong> name of person/people who performed the task</strong>
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
-:* e.g. read through papers, worked on proposal, etc.
 = Discussion =

Team:Hawaii/Notebook/2008-07- 2

From 2008.igem.org