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Team:Hawaii/Notebook/2008-07- 8
From 2008.igem.org
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===Completed oligonucleotide experiment=== | ===Completed oligonucleotide experiment=== | ||
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
| - | + | [[Image:C0012_plasmid_prep.JPG|thumb|right|The two expected bands corresponding to the vector and the BioBrick insert are clearly visible. First "good" plasmid prep!]] | |
:* Ran RE digested ligations on 4% gel. Too low DNA concentration so no ligated products were seen. | :* Ran RE digested ligations on 4% gel. Too low DNA concentration so no ligated products were seen. | ||
:* Redid ligation and RE digest. Loaded 20 μl in 4% gel. | :* Redid ligation and RE digest. Loaded 20 μl in 4% gel. | ||
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:* Extracted BBa_E0240 and BBa_I14032 using commercial grade DH5α super competent cells. | :* Extracted BBa_E0240 and BBa_I14032 using commercial grade DH5α super competent cells. | ||
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= Discussion = | = Discussion = | ||
Revision as of 01:19, 10 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Completed oligonucleotide experiment
- Grace
- Ran RE digested ligations on 4% gel. Too low DNA concentration so no ligated products were seen.
- Redid ligation and RE digest. Loaded 20 μl in 4% gel.
- RE digested C0012.
- Gel extraction of C0012 vector and ligated products.
- Ligated C0012 vector with pnir, slr2016, and pilA.
Biobrick extraction
- Krystle
- Extracted BBa_E0240 and BBa_I14032 using commercial grade DH5α super competent cells.
Discussion
Quote of the Day
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
