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Minnesota/10 July 2008
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|1. '''Dephosphorylation:''' Continued with double digestion base vector product from 07-09-2008. Used Antartic phosphoylase to dephosphorylate the base vector. Want to dephosphorylate the base vector to prevent it from closing - want it to stay a linear digested product. | |1. '''Dephosphorylation:''' Continued with double digestion base vector product from 07-09-2008. Used Antartic phosphoylase to dephosphorylate the base vector. Want to dephosphorylate the base vector to prevent it from closing - want it to stay a linear digested product. | ||
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{|border="1" align="left" | {|border="1" align="left" | ||
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| - | !| Gene !!10x Buffer | + | !| Gene !!10x Buffer !!DNA !!Phosphotase |
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| - | |Base Vector ||5.6uL | + | |Base Vector ||5.6uL ||50.0uL ||1.0uL |
|- | |- | ||
|} | |} | ||
Revision as of 16:05, 10 July 2008
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| 1. Dephosphorylation: Continued with double digestion base vector product from 07-09-2008. Used Antartic phosphoylase to dephosphorylate the base vector. Want to dephosphorylate the base vector to prevent it from closing - want it to stay a linear digested product. |
| Gene | 10x Buffer | DNA | Phosphotase |
|---|---|---|---|
| Base Vector | 5.6uL | 50.0uL | 1.0uL |
