Minnesota/11 July 2008

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|1. '''Ligation Transformations SUCCESSFUL''': 3 out of 4 plates had growth.
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|2. '''Pick Colonies from Transformation Plates:''' Place picked colonies in 2mL LB cultures. Allow 2 hours to incubate and grow @ 37C with shaking @ 225rpm.
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|3. '''Plasmid Prep''': Plasmid prep the 2mL cultures of ligation products. This is done to extract DNA. 
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|4. '''Plasmid Prep Promoters and Terminators'''
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|5. '''Post Spectrophotometry Results on Wiki Notebook Page'''
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Latest revision as of 16:20, 11 July 2008

Back to Notebook Home
Go to Previous Day (July 10)Go to Next Day (July 14)
1. Ligation Transformations SUCCESSFUL: 3 out of 4 plates had growth.
2. Pick Colonies from Transformation Plates: Place picked colonies in 2mL LB cultures. Allow 2 hours to incubate and grow @ 37C with shaking @ 225rpm.
3. Plasmid Prep: Plasmid prep the 2mL cultures of ligation products. This is done to extract DNA.
4. Plasmid Prep Promoters and Terminators
5. Post Spectrophotometry Results on Wiki Notebook Page