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Minnesota/11 July 2008
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Emartin9808 (Talk | contribs) (New page: {|style="align:left" width="965" |- |''' Back to Notebook Home''' |- |'''Go to Previous Day (July 10)'''|| width=158|'''[[...) |
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|'''[[Team:Minnesota/NotebookComparator| Back to Notebook Home]]''' | |'''[[Team:Minnesota/NotebookComparator| Back to Notebook Home]]''' | ||
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| - | |'''[[Minnesota/10 July 2008|Go to Previous Day (July 10)]]'''|| width=158|'''[[Minnesota/ | + | |'''[[Minnesota/10 July 2008|Go to Previous Day (July 10)]]'''|| width=158|'''[[Minnesota/14 July 2008|Go to Next Day (July 14)]]''' |
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| + | |1. '''Ligation Transformations SUCCESSFUL''': 3 out of 4 plates had growth. | ||
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| + | |2. '''Pick Colonies from Transformation Plates:''' Place picked colonies in 2mL LB cultures. Allow 2 hours to incubate and grow @ 37C with shaking @ 225rpm. | ||
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| + | |3. '''Plasmid Prep''': Plasmid prep the 2mL cultures of ligation products. This is done to extract DNA. | ||
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| + | |4. '''Plasmid Prep Promoters and Terminators''' | ||
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| + | |5. '''Post Spectrophotometry Results on Wiki Notebook Page''' | ||
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|} | |} | ||
Latest revision as of 16:20, 11 July 2008
| Back to Notebook Home | |
| Go to Previous Day (July 10) | Go to Next Day (July 14) |
| 1. Ligation Transformations SUCCESSFUL: 3 out of 4 plates had growth. |
| 2. Pick Colonies from Transformation Plates: Place picked colonies in 2mL LB cultures. Allow 2 hours to incubate and grow @ 37C with shaking @ 225rpm. |
| 3. Plasmid Prep: Plasmid prep the 2mL cultures of ligation products. This is done to extract DNA. |
| 4. Plasmid Prep Promoters and Terminators |
| 5. Post Spectrophotometry Results on Wiki Notebook Page |
