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Team:Hawaii/Notebook/2008-07-11
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep of Biobricks=== :<strong> Krystle</strong> :* Plasmid prep for B0015, E0026, R0010, J33207, B0030 ===Con...) |
(→Construction of pnir, slr2016, pilA with vector) |
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::* Ligated C0012 with vector as positive control | ::* Ligated C0012 with vector as positive control | ||
:* Ligated annealed products with vector | :* Ligated annealed products with vector | ||
| - | :* Ran ligations on gel | + | :* Ran ligations on [https://2008.igem.org/Image:071108_vector_ligations.jpg gel] |
::* Annealed products + vector = smear | ::* Annealed products + vector = smear | ||
| - | ::* Rest of gel blank = not enough DNA | + | ::* Rest of gel blank = not enough DNA |
===Made stock solutions=== | ===Made stock solutions=== | ||
| Line 33: | Line 33: | ||
:<strong> Margaret</strong> | :<strong> Margaret</strong> | ||
| + | ===OriT Ligation Continued=== | ||
| + | :<strong> Margaret</strong> | ||
| + | |||
| + | :*digested ligation with XbaI amd PstI, ran gel, cut it out (no picture). It is sitting in the 4°C until Monday. | ||
= Discussion = | = Discussion = | ||
Latest revision as of 09:06, 14 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep of Biobricks
- Krystle
- Plasmid prep for B0015, E0026, R0010, J33207, B0030
Construction of pnir, slr2016, pilA with vector
- Grace
- Extracted DNA from gel using Qiagen MiniElute Gel Extraction Kit
- Ligated pnir, slr2016, pilA with BBa_C0012 harboring vector
- Ligated C0012 with vector as positive control
- Ligated annealed products with vector
- Ran ligations on gel
- Annealed products + vector = smear
- Rest of gel blank = not enough DNA
Made stock solutions
- Grace
- For plasmid prep:
- GTE
- NaOH/SDS
- Potassium Acetate
- General:
- 0.25 M D-glucose
- Autoclaved nanopure water
Made DB3.1 competent cells
- Margaret
OriT Ligation Continued
- Margaret
- digested ligation with XbaI amd PstI, ran gel, cut it out (no picture). It is sitting in the 4°C until Monday.
Discussion
Quote of the Day
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
