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Team:Hawaii/Notebook/2008-07-17
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Colony PCR=== :<strong> Grace </strong> ===pSB3K3 plasmid prep=== :<strong> Margaret </strong> == Drylab Work == ===...) |
(→Colony PCR) |
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| Line 3: | Line 3: | ||
= Things we did today = | = Things we did today = | ||
== Wetlab work == | == Wetlab work == | ||
| - | === | + | ===PCR=== |
:<strong> Grace </strong> | :<strong> Grace </strong> | ||
| + | |||
| + | :* Colony PCR for GFP fusion brick and new pRL1383a to verify inserts | ||
| + | :* Sequencing (25 mu;l reactions) | ||
| + | ::* ''nir'' promoter | ||
| + | ::* ''slr2016'' | ||
| + | ::* ''pilA'' | ||
| + | ::* BBa_B0015 | ||
| + | ::* BBa_B0024 | ||
| + | ::* BBa_B0030 | ||
| + | ::* BBa_C0012 | ||
| + | ::* BBa_E0040 | ||
| + | ::* BBa_J04430 | ||
| + | ::* BBa_J33207 | ||
| + | ::* BBa_R0010 | ||
| + | :* Ran all PCR products on a 4% gel to check | ||
| + | ::* Gel ran funny -- loading dye ran 2x as fast as the DNA itself | ||
| + | ::* Poor ladder resolution on gel | ||
| + | ::* No bands seen for GFP fusion, pRL1383a, R0010 | ||
| + | ::* Incorrect bands for B0015 and J33207 | ||
| + | ::* Will redo plasmid preps for R0010, B0015, J33207 | ||
| + | ::* Will redo PCR reaction tomorrow for pRL1383a, GFP fusion, B0015, J33207, R0010 | ||
| + | |||
| + | === Plasmid Prep=== | ||
| + | :<strong> Grace <strong> | ||
| + | |||
| + | :* Inoculated 7 ml LB + amp<sub>100<sub> with single colony of B0015, R0010, J04430 for redo of plasmid prep tomorrow | ||
| + | |||
| + | === Restreak to Purify=== | ||
| + | :<strong>Grace<strong> | ||
| + | |||
| + | :* Restreaked GFP fusion and pRL1383a (MCS replaced) transformants to purify | ||
===pSB3K3 plasmid prep=== | ===pSB3K3 plasmid prep=== | ||
Revision as of 03:38, 18 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
PCR
- Grace
- Colony PCR for GFP fusion brick and new pRL1383a to verify inserts
- Sequencing (25 mu;l reactions)
- nir promoter
- slr2016
- pilA
- BBa_B0015
- BBa_B0024
- BBa_B0030
- BBa_C0012
- BBa_E0040
- BBa_J04430
- BBa_J33207
- BBa_R0010
- Ran all PCR products on a 4% gel to check
- Gel ran funny -- loading dye ran 2x as fast as the DNA itself
- Poor ladder resolution on gel
- No bands seen for GFP fusion, pRL1383a, R0010
- Incorrect bands for B0015 and J33207
- Will redo plasmid preps for R0010, B0015, J33207
- Will redo PCR reaction tomorrow for pRL1383a, GFP fusion, B0015, J33207, R0010
Plasmid Prep
- Grace <strong>
- Inoculated 7 ml LB + amp100 with single colony of B0015, R0010, J04430 for redo of plasmid prep tomorrow
Restreak to Purify
- <strong>Grace<strong>
- Restreaked GFP fusion and pRL1383a (MCS replaced) transformants to purify
pSB3K3 plasmid prep
- <strong> Margaret </strong>
Drylab Work
Name of Task
- <strong> name of person/people who performed the task</strong>
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
