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Team:Virginia/Protocols
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| + | ==Electrophoresis Gel== | ||
| + | *In a 100 mL Erlenmeyer flask, place and pour: 0.5 g Agar and 50 mL 1X TAE Buffer | ||
| + | *Microwave for 30 seconds and then swirl the flask | ||
| + | *Microwave for 15 seconds (caution: do not let the solution boil out of the flask) | ||
| + | *Pipette 2 microliters of Ethidium Bromide into the gel solution and then swirl flask | ||
| + | **Ethidium Bromide stains the plate for DNA to be observed under UV light | ||
| + | *NOTE: Ethidium Bromide is a hazardous chemical and must be disposed of properly - this includes the gel itself | ||
Revision as of 14:48, 18 July 2008
Hello World
Electrophoresis Gel
- In a 100 mL Erlenmeyer flask, place and pour: 0.5 g Agar and 50 mL 1X TAE Buffer
- Microwave for 30 seconds and then swirl the flask
- Microwave for 15 seconds (caution: do not let the solution boil out of the flask)
- Pipette 2 microliters of Ethidium Bromide into the gel solution and then swirl flask
- Ethidium Bromide stains the plate for DNA to be observed under UV light
- NOTE: Ethidium Bromide is a hazardous chemical and must be disposed of properly - this includes the gel itself
