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User contributions
From 2008.igem.org
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- 13:53, 7 August 2008 (diff | hist) Virginia/6 August 2008 (top)
- 14:47, 6 August 2008 (diff | hist) N Virginia/6 August 2008 (New page: ==TODO== *Miniprep the new overnight broths of the assemblies trying to redo the *Digest the miniprepped DNA *Gel the digested DNA *If bands come up as expected, run a nusieve gel to extr...)
- 14:38, 6 August 2008 (diff | hist) Virginia/4 August 2008 (→Accomplished) (top)
- 14:37, 6 August 2008 (diff | hist) N Virginia/5 August 2008 (New page: ==TODO== *Re-gel the digested parts to cut out parts and carry on with the second stage of assemblies ==Results== *second running of gel did not show any different results. Some digested ...) (top)
- 13:55, 6 August 2008 (diff | hist) N Virginia/4 August 2008 (New page: ==Goals== *Assemblies 51-57 **Digest for standard assembly **Use low melt gel to purify parts ==Accomplished== *Gel of assemblies not as expected **Potential bands of small cutouts not vi...)
- 13:48, 6 August 2008 (diff | hist) N Virginia/1 August 2008 (New page: ==Goals== *Assemblies 46-50 **Plan to use low melt gel to separate parts **Standard assembly ==Accomplished== *Difficulties with the low-melt gel **Bands are crooked, not the right length...) (top)
- 19:27, 28 July 2008 (diff | hist) Team:Virginia/Protocols
- 21:34, 22 July 2008 (diff | hist) m Team:Virginia/Protocols (→Electrophoresis Gel (Making))
- 21:33, 22 July 2008 (diff | hist) Team:Virginia/Protocols (→3A assembly Ligation)
- 20:36, 22 July 2008 (diff | hist) Team:Virginia/Protocols (→Gel Electrophoresis (Loading & Running))
- 20:52, 21 July 2008 (diff | hist) Team:Virginia/Protocols (→Electrophoresis Gel)
- 20:26, 21 July 2008 (diff | hist) Team:Virginia/Protocols (→Electrophoresis Gel)
- 18:03, 21 July 2008 (diff | hist) m Virginia/21 July 2008 (top)
- 18:02, 21 July 2008 (diff | hist) m Virginia/21 July 2008 (→Where We Stand)
- 18:00, 21 July 2008 (diff | hist) Virginia/21 July 2008
- 17:49, 21 July 2008 (diff | hist) N Virginia/21 July 2008 (New page: ==Where We Stand== *'''Genetic Attenuator''' *Measurement Plasmid **Activating Promoter with Arabinos X'''incomplete''' **Suspicion that measurement plasmid may not work in DB3.1 cells we...)
- 17:26, 21 July 2008 (diff | hist) m Virginia/19 July 2008 (top)
- 15:00, 18 July 2008 (diff | hist) Team:Virginia/Protocols (→Electrophoresis Gel)
- 14:48, 18 July 2008 (diff | hist) Team:Virginia/Protocols
- 14:38, 18 July 2008 (diff | hist) Nm Team:Virginia/Protocols (New page: Hello World)
- 14:34, 18 July 2008 (diff | hist) Virginia/18 July 2008 (top)
- 14:04, 18 July 2008 (diff | hist) Virginia/18 July 2008 (→Overnight Results)
- 14:00, 18 July 2008 (diff | hist) Virginia/18 July 2008 (→Overnight)
- 14:00, 18 July 2008 (diff | hist) N Virginia/18 July 2008 (New page: ==Overnight== *plates A11() A12 and A13 *The screaning plasmid grew in overnight broth but we could not induce it to fluoresce *Overnight broths of BP2, BP3, PSB1A10, psb1A10 with arabinos...)
- 15:14, 17 July 2008 (diff | hist) Virginia/17 July 2008 (→Goals) (top)
- 15:12, 17 July 2008 (diff | hist) Virginia/17 July 2008 (→Goals)
- 15:09, 17 July 2008 (diff | hist) Virginia/17 July 2008 (→Goals)
- 14:59, 17 July 2008 (diff | hist) Virginia/17 July 2008 (→Goals)
- 14:50, 17 July 2008 (diff | hist) Virginia/17 July 2008 (→Goals)
- 14:27, 17 July 2008 (diff | hist) N Virginia/17 July 2008 (New page: ==Overnight Results== *only plate with R0010 +A4 grew which is the same result as from the night before **Which leads us to think our assemblies have some problem *the assembly of BP2 and ...)
- 14:57, 15 July 2008 (diff | hist) N Virginia/15 July 2008 (New page: ==Goals== *GET GFP EXPRESSION! *miniprep DNA **Screening plasmid (psb1A10) **Assembly 3 (I715039 and E0240) **BP 2 **BP 3 *Assemble parts **Repressible Promoter(R0040) and GFP(E0240) **Sta...) (top)
- 14:46, 15 July 2008 (diff | hist) Virginia/14 July 2008 (top)
- 19:34, 14 July 2008 (diff | hist) N Virginia/14 July 2008 (New page: ==Goals== *turn in time sheets! *Examine flourescent proteins under flourescence camera **psb1A10 **assembly 3 of i715039 and E0240 *make overnight broths of these flourescent protein cell...)
- 19:23, 14 July 2008 (diff | hist) Virginia/11 July 2008 (top)
- 14:40, 8 July 2008 (diff | hist) N Virginia/8 July 2008 (New page: ==Goals== *Prepare overnight broth of successful single colony of Promoter + RBS transformation *Plate Screening plasmid (psb1a10) when it arrives from iGem *Try again with ligation of RFP...)
- 14:26, 8 July 2008 (diff | hist) N Virginia/7 July 2008 (New page: ==Goals== *Buy plane tickets *Reserve hotel room *Promoter + RBS ligation *Promoter + RBS transformation and plating ==Results== *have been having problems with purification of small part...) (top)
- 14:52, 1 July 2008 (diff | hist) N Virginia/1 July 2008 (New page: ==Goals== *digest parts *do not purify parts *heat deactivate digestion enzyme *ligate *transform Notebook) (top)
- 14:49, 1 July 2008 (diff | hist) N Virginia/27 June 2008 (New page: ==Goals== *small broth of plated parts *minipreps of parts that are going to be assembled *redigest all the assembly parts *run gels to verify purified cut outs *if possible run 3A assembl...) (top)
- 14:39, 30 June 2008 (diff | hist) N Virginia/28 June 2008 (New page: *Take out incubated over night brothes *spin down cultures to pellets to store in freezer Notebook) (top)
- 14:37, 30 June 2008 (diff | hist) N Virginia/30 June 2008 (New page: ==Goals== *Order midiprep kit *miniprep ligation of BP1 and terminator (Assembly 2) *Digest ligation *run regular gel of ligation and large purified parts that will be used in other ligati...)
- 15:35, 27 June 2008 (diff | hist) m Virginia/26 June 2008 (top)
- 15:29, 27 June 2008 (diff | hist) Virginia/26 June 2008
- 15:26, 27 June 2008 (diff | hist) m Virginia/26 June 2008 (→Notes)
- 15:25, 27 June 2008 (diff | hist) N Virginia/26 June 2008 (New page: ==Goals== *small broth of plated terminator *large broth of E0240(RFP) and I715039(GFP) *mini prepe PRE RFP and RET GFP for another ligation *redigest 6 parts for the three ligations *pu...)
- 15:13, 27 June 2008 (diff | hist) Virginia/25 June 2008 (top)
- 14:33, 25 June 2008 (diff | hist) Virginia/25 June 2008
- 14:32, 25 June 2008 (diff | hist) Virginia/25 June 2008
- 14:32, 25 June 2008 (diff | hist) Virginia/25 June 2008
- 14:31, 25 June 2008 (diff | hist) Virginia/25 June 2008
- 14:28, 25 June 2008 (diff | hist) N Virginia/5 July 2008 (New page: 50% Off Fireworks!!! Notebook) (top)
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