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Team:The University of Alberta/18 July 2008
From 2008.igem.org
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(New page: ===Today=== *Got the sequencing back that Winnie did on the BisDA gene: it was LLC because she used the primers; no big deal though because the size was correct and we're pretty sure that ...) |
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*Got the sequencing back that Winnie did on the BisDA gene: it was LLC because she used the primers; no big deal though because the size was correct and we're pretty sure that it's BisDA | *Got the sequencing back that Winnie did on the BisDA gene: it was LLC because she used the primers; no big deal though because the size was correct and we're pretty sure that it's BisDA | ||
*Began westerns on the total/soluable crude butanol proteins | *Began westerns on the total/soluable crude butanol proteins | ||
| + | *Digested Tryp and J6 with Xba and Pst. | ||
| + | **Ligated them together, left the reaction to go overnight. Will transform tomorrow. | ||
| + | *Did mni-preps of all biobricks: ER01, ER02, BisDA, BisDB, Lac1 ERE, TDNA-MCS, RBS-6x His, TetR-RBS-6x His. | ||
| + | **Double digested the above parts with Xbal/Pst. Gel didnot turn out good- Possibly due to water contamination, and also only one enzyme worked in each case. Redoing digestions on Monday. | ||
Latest revision as of 22:56, 18 July 2008
Today
- Got the sequencing back that Winnie did on the BisDA gene: it was LLC because she used the primers; no big deal though because the size was correct and we're pretty sure that it's BisDA
- Began westerns on the total/soluable crude butanol proteins
- Digested Tryp and J6 with Xba and Pst.
- Ligated them together, left the reaction to go overnight. Will transform tomorrow.
- Did mni-preps of all biobricks: ER01, ER02, BisDA, BisDB, Lac1 ERE, TDNA-MCS, RBS-6x His, TetR-RBS-6x His.
- Double digested the above parts with Xbal/Pst. Gel didnot turn out good- Possibly due to water contamination, and also only one enzyme worked in each case. Redoing digestions on Monday.
