User:University of Washington/18 July 2008

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== Lambda Red Recombineering of RP4 (Bryan) ==
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Continued troubleshooting of PCR.  Since all previous attempts to PCR have failed, attempted a control reaction using standard BioBrick primers on a part which has been both transformed and sequenced by our team.
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Made glycerol stock of BW25141/pKD78.  Mini-prepped pKD78 x2. Only the second mini-prep electrophoresed as expected (~6 kb), first mini-prep may be compromised.  Now have Lambda Red recombinatory expression vector--some positive news.
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== AHL synthesis in yeast (Bryan) ==
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Attempted to rescue part C0061 from both 2007 & 2008 distribution by PCR amplification using standard BioBrick primers.
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Klavins lab had success transforming part C0161.  Obtained an ON broth culture.
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Back to [[Team:University_of_Washington/Notebook#Notebook]]
Back to [[Team:University_of_Washington/Notebook#Notebook]]

Latest revision as of 23:10, 21 July 2008

Lambda Red Recombineering of RP4 (Bryan)

Continued troubleshooting of PCR. Since all previous attempts to PCR have failed, attempted a control reaction using standard BioBrick primers on a part which has been both transformed and sequenced by our team.

Made glycerol stock of BW25141/pKD78. Mini-prepped pKD78 x2. Only the second mini-prep electrophoresed as expected (~6 kb), first mini-prep may be compromised. Now have Lambda Red recombinatory expression vector--some positive news.

AHL synthesis in yeast (Bryan)

Attempted to rescue part C0061 from both 2007 & 2008 distribution by PCR amplification using standard BioBrick primers.

Klavins lab had success transforming part C0161. Obtained an ON broth culture.


Back to Team:University_of_Washington/Notebook#Notebook

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