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Team:Hawaii/Notebook/2008-07-21
From 2008.igem.org
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:*continued from [[Team:Hawaii/Notebook/2008-07-19|Saturday]], ran a gel | :*continued from [[Team:Hawaii/Notebook/2008-07-19|Saturday]], ran a gel | ||
| - | + | ===Restriction Digest=== | |
| + | :<strong>Krystle</strong> | ||
| + | :*Performed a 2 separate restriction digests on nir, gfp, and gfp fusion brick with EcoRI and SpeI | ||
| + | ::''trial 1:'' digested 5ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 3 hours | ||
| + | ::''trial 2:'' digested 25ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 2 hours | ||
== Drylab Work == | == Drylab Work == | ||
Revision as of 04:18, 22 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Colony PCR
- Margaret
- Colony PCR of pSB1A2, pSB1A3, pSB3K3
PCR amplification of pRL1383a parts
- continued from Saturday, ran a gel
Restriction Digest
- Krystle
- Performed a 2 separate restriction digests on nir, gfp, and gfp fusion brick with EcoRI and SpeI
- trial 1: digested 5ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 3 hours
- trial 2: digested 25ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 2 hours
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
