Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)

Revision as of 18:43, 22 July 2008

PCR products

PCR products
Number	Name	Oligo F	Oligo R	Matrix	Length	Comments	Product Left ?	[]ng/µL	Date
									[[ ]]
									[[ ]]

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number	Name	Biobricks	Description	Products in use	Date
MP1	[http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002]		Strong constitutive promoter in J61002		2007
MP2	[http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002]		Medium constitutive promoter in J61002	MP2.2	2007
MP3	[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3]		Double terminator	MP3.3, MP3.4	2007
MP4	[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3]		AraC pBAD		2007
MP5	[http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]		Strong RBS (Efficiency = 0,6)		2007
MP6	[http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]		ECFP (RBS+LVA+Term)	MP6'.1, MP6'.2	2007
MP8	[http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]		gfp tri-part; strong rbs		2007

Plasmids: MiniPrep "in preparation"
Number	Name	Biobricks	Description	Products in use	Date
	[http://partsregistry.org/Part:BBa_B0034 B0034]		Strongest RBS (Efficiency = 1)		2008
	[http://partsregistry.org/Part:BBa_C0040 C0040]		tetR repressor		2008
	[http://partsregistry.org/Part:BBa_C0079 C0079]		lasR activator with LVA Tag		2008
	[http://partsregistry.org/Part:BBa_C0179 C0179]		lasR activator		2007
	[http://partsregistry.org/Part:BBa_E0030 E0030]		YFP		2008
	[http://partsregistry.org/Part:BBa_E0040 E0040]		GFP		2008
	[http://partsregistry.org/Part:BBa_E1010 E1010]		mRFP		2008
	[http://partsregistry.org/Part:BBa_J23101 J23101]		Strongest Constitutive promoter		2008
	[http://partsregistry.org/Part:BBa_J23109 J23109]		Weak Constitutive promoter		2008
	[http://partsregistry.org/Part:BBa_R0040 R0040]		tetR repressible promoter		2008
	[http://partsregistry.org/Part:BBa_R0079 R0079]		promoter pLas (lasr & PAI regulated)		2008
	[http://partsregistry.org/Part:BBa_S03154 S03154]		B0034(rbs) ->LasI		2008
	[http://partsregistry.org/Part:BBa_S03879 S03879]		B0034(rbs) -> TetR		2008

Digestion Products

(Legend: Vector, Insert)

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Size	Description	Product Left	[]ng/µL	Date
D1	<bbpart>pJ23100</bbpart>	<bbpart>pJ23100</bbpart>	Spe1	Pst1	2096	Vector with the J23100 promoter ready for insertion of a RBS/CDS insert	Ø (24/09/07)	34	July 17
D2	<bbpart>mRFP dblTer</bbpart>	<bbpart>pJ23100</bbpart>	Spe1	Pst1	887			27	July 17

@@ Line 59: / Line 59: @@
 |align="center"|[http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]]
-|align="center"|Strong constitutive promoter in J61002 (clones 1 + 2)
+|align="center"|Strong constitutive promoter in J61002
 |
 |align="center"|2007
@@ Line 66: / Line 66: @@
 |align="center"|[http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]]
-|align="center"|Medium constitutive promoter in J61002 (clones 1 + 2)
+|align="center"|Medium constitutive promoter in J61002
 |align="center"|MP2.2
 |align="center"|2007
@@ Line 73: / Line 73: @@
 |align="center"|[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3]
 |align="center"|[[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-|align="center"|Double terminator (clones 1 + 2)
+|align="center"|Double terminator
 |align="center"|MP3.3, MP3.4
 |align="center"|2007
@@ Line 80: / Line 80: @@
 |align="center"|[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3]
 |align="center"|[[Image:Part_icon_regulatory.png]]
-|align="center"|AraC pBAD (clones 1 + 2)
+|align="center"|AraC pBAD
 |
 |align="center"|2007
@@ Line 87: / Line 87: @@
 |align="center"|[http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]]
-|align="center"|Strong RBS (clones 1 + 2)
+|align="center"|Strong RBS (Efficiency = 0,6)
 |
 |align="center"|2007
@@ Line 94: / Line 94: @@
 |align="center"|[http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
-|align="center"|ECFP (RBS+LVA+Term) (clones 1 + 2)
+|align="center"|ECFP (RBS+LVA+Term)
 |align="center"|MP6'.1, MP6'.2
 |align="center"|2007
@@ Line 101: / Line 101: @@
 |align="center"|[http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
-|align="center"|gfp tri-part; strong rbs (clones 1 + 2)
+|align="center"|gfp tri-part; strong rbs
 |
 |align="center"|2007
@@ Line 122: / Line 122: @@
 |align="center"|[http://partsregistry.org/Part:BBa_B0034 B0034]
 |align="center"|[[Image:Part_icon_rbs.png]]
-|align="center"|Strongest RBS
+|align="center"|Strongest RBS (Efficiency = 1)
 |
 |align="center"|2008