Team:The University of Alberta/23 July 2008

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(Difference between revisions)
(New page: *Gel purification of 1) conc. double digests of BisdA and BisdB with both (NgoMIV+Age1) and (XbaI+PstI); 2)conc. digest of vector containing the TetR binding site and the RBS with NgoMIV ...)
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*Gel purification of 1) conc. double digests of BisdA and BisdB with both (NgoMIV+Age1) and (XbaI+PstI);  
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*Gel purification of 1) conc. double digests of BisdA and BisdB with both (NgoMIV+Age1) and (XbaI+PstI); 2)conc. digest of vector containing the TetR binding site and the RBS with NgoMIV (digest was treated with Antartic Phosphatase; 3) conc. digest of J61003 with (SpeI+PstI) (digest was treated with Antartic Phosphatase).
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2)conc. digest of vector containing the TetR binding site and the RBS with NgoMIV (digest was treated with Antartic Phosphatase; 3) conc. digest of J61003 with (SpeI+PstI) (digest was treated with Antartic Phosphatase).
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Revision as of 15:43, 23 July 2008

  • Gel purification of 1) conc. double digests of BisdA and BisdB with both (NgoMIV+Age1) and (XbaI+PstI); 2)conc. digest of vector containing the TetR binding site and the RBS with NgoMIV (digest was treated with Antartic Phosphatase; 3) conc. digest of J61003 with (SpeI+PstI) (digest was treated with Antartic Phosphatase).
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