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Team:The University of Alberta/23 July 2008
From 2008.igem.org
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| - | *Gel purification of 1) conc. double digests of BisdA and BisdB with both (NgoMIV+Age1) and (XbaI+PstI); 2)conc. digest of vector containing the TetR binding site and the RBS with NgoMIV (digest was treated with Antartic Phosphatase; 3) conc. digest of J61003 with (SpeI+PstI) (digest was treated with Antartic Phosphatase). | + | *Gel purification of 1) conc. double digests of BisdA and BisdB with both (NgoMIV+Age1) and (XbaI+PstI); 2)conc. digest of vector containing the TetR binding site and the RBS with NgoMIV (digest was treated with Antartic Phosphatase); 3) conc. digest of J61003 with (SpeI+PstI) (digest was treated with Antartic Phosphatase). |
Revision as of 15:44, 23 July 2008
- Gel purification of 1) conc. double digests of BisdA and BisdB with both (NgoMIV+Age1) and (XbaI+PstI); 2)conc. digest of vector containing the TetR binding site and the RBS with NgoMIV (digest was treated with Antartic Phosphatase); 3) conc. digest of J61003 with (SpeI+PstI) (digest was treated with Antartic Phosphatase).
