Minnesota/2 July 2008

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Revision as of 18:27, 23 July 2008

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Plasmid prep base vectors

1. Miraculously receive DB3.1 E.Coli cells

2. Transformation: Transform the plasmid base vector containing ccdB gene into DB3.1 E. Coli. The DB3.1 E. Coli strain is resistant to the toxin produced by the ccdB gene, so we transform the base vector into this to amplify the amount of plasmid DNA.

3. Plasmid prep: Extract the DNA/plasmid with ccdB gene from DB3.1 E. Coli. Will have a large amount of plasmid base vectors containing ccdB gene, and will then double digest to cut out the ccdB gene at it's restriction sites.

@@ Line 9: / Line 9: @@
 |-
-|'''1. Miraculously receive DB3.1 E.Coli base vectors'''
+|'''1. Miraculously receive DB3.1 E.Coli cells'''
 |-
-|'''2. Plasmid prep:''' Extract the DNA/plasmid with ccdb gene from E. Coli.
+|'''2. Transformation:''' Transform the plasmid base vector containing ccdB gene into DB3.1 E. Coli. The DB3.1 E. Coli strain is resistant to the toxin produced by the ccdB gene, so we transform the base vector into this to amplify the amount of plasmid DNA.
 |-
 [[Image:plasmidprep.JPG|600px||center|thumb|Plasmid prep base vectors]]
 |-
-|'''3. Transformation:''' Transform DB3.1 E.Coli
+|'''3. Plasmid prep:''' Extract the DNA/plasmid with ccdB gene from DB3.1 E. Coli. Will have a large amount of plasmid base vectors containing ccdB gene, and will then double digest to cut out the ccdB gene at it's restriction sites.
 |}