Minnesota/2 July 2008

From 2008.igem.org

(Difference between revisions)

Latest revision as of 21:17, 23 July 2008

Back to Notebook Home
Go to Previous Day (July 1)	Go to Next Day (July 3)

1. Miraculously receive DB3.1 E.Coli cells

2. Transformation: Transform the plasmid base vector containing ccdB gene into DB3.1 E. Coli. The DB3.1 E. Coli strain is resistant to the toxin produced by the ccdB gene, so we transform the base vector into this to amplify the amount of plasmid DNA.

3. Plasmid prep: Extract the DNA/plasmid with ccdB gene from DB3.1 E. Coli. Will have a large amount of plasmid base vectors containing ccdB gene, and will then double digest to cut out the ccdB gene at it's restriction sites.

@@ Line 9: / Line 9: @@
 |-
-|'''1. Miraculously receive DB3.1 E.Coli base vectors'''
+|'''1. Miraculously receive DB3.1 E.Coli cells'''
 |-
-|'''2. Plasmid prep:''' Extract the DNA/plasmid with ccdb gene from E. Coli.
+|'''2. Transformation:''' Transform the plasmid base vector containing ccdB gene into DB3.1 E. Coli. The DB3.1 E. Coli strain is resistant to the toxin produced by the ccdB gene, so we transform the base vector into this to amplify the amount of plasmid DNA.
 |-
-[[Image:plasmidprep.JPG|600px||center|thumb|Plasmid prep base vectors]]
+|[[Image:plasmidprep.JPG|600px||center|thumb|Plasmid prep base vectors]]
 |-
-|'''3. Transformation:''' Transform DB3.1 E.Coli
+|'''3. Plasmid prep:''' Extract the DNA/plasmid with ccdB gene from DB3.1 E. Coli. Will have a large amount of plasmid base vectors containing ccdB gene, and will then double digest to cut out the ccdB gene at it's restriction sites.
 |}