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Team:Hawaii/Notebook/2008-07-29
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:* attempt to pipette out DNA as it runs into the second well | :* attempt to pipette out DNA as it runs into the second well | ||
:* no more gel cutting??? | :* no more gel cutting??? | ||
| + | |||
| + | ===PCR amplification of pRL1383a parts=== | ||
| + | :<strong>Margaret</strong> | ||
| + | |||
| + | :* Made a reaction buffer containing {38.5ul, 20ul accusure, 10ul dNTPs, & 5ul 5XBuffer}. This should be good for 20 50ul reactions. | ||
| + | |||
| + | :*Amplified with Accusure: aadA ([http://partsregistry.org/Part:BBa_J23012 BBa_J23012]), aadaA (pRL1383a), rep region, oriV | ||
| + | :*Amplified with Green Taq: rep region | ||
| + | |||
| + | ===Media=== | ||
| + | |||
| + | :*started to make LB amp100 plates, but autoclave room is closed. Remember to do it tomorrow. | ||
== Drylab Work == | == Drylab Work == | ||
Revision as of 03:42, 30 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Gel purification of ccdB PCR product
- Grace
RE digests
- Grace
- Digested pRL1383a (from last night) with BamHI
- Digested B0030 and B0024 (from last night) with EcoRI
- Sequentially digested gel purified ccdB with HindIII and BamHI
Construction of BioBricks
- Grace
- Purified RE digests on EtBr stained 3% agarose gel; extracted desired bands
- Ligated pRL1383a with ccdB, nir with B0030, and GFP and GFPf with B0024
Transformation of constructs in DB3.1
- Grace
Direct Band Extraction Method
- make two comb gels
- run test UPA band along with ladder
- attempt to pipette out DNA as it runs into the second well
- no more gel cutting???
PCR amplification of pRL1383a parts
- Margaret
- Made a reaction buffer containing {38.5ul, 20ul accusure, 10ul dNTPs, & 5ul 5XBuffer}. This should be good for 20 50ul reactions.
- Amplified with Accusure: aadA ([http://partsregistry.org/Part:BBa_J23012 BBa_J23012]), aadaA (pRL1383a), rep region, oriV
- Amplified with Green Taq: rep region
Media
- started to make LB amp100 plates, but autoclave room is closed. Remember to do it tomorrow.
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
LOL! http://www.youtube.com/watch?v=J0s0Y3-BCaw
