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Illinois/17 July 2008
From 2008.igem.org
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(New page: '''Antibody/GPCR Fusion''' *Consulting meeting with Huimin Zhao (Associate Professor, Chemical and Biomolecular Engineering)) |
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'''Antibody/GPCR Fusion''' | '''Antibody/GPCR Fusion''' | ||
*Consulting meeting with Huimin Zhao (Associate Professor, Chemical and Biomolecular Engineering) | *Consulting meeting with Huimin Zhao (Associate Professor, Chemical and Biomolecular Engineering) | ||
| + | |||
| + | '''LAB WORK''' | ||
| + | |||
| + | '''Receptor Tyrosine Kinase method''' | ||
| + | |||
| + | E.Coli Media | ||
| + | |||
| + | LB medium, per liter | ||
| + | |||
| + | 10g tryptone | ||
| + | |||
| + | 5g yeast extract | ||
| + | |||
| + | 5g NaCl | ||
| + | |||
| + | 1 mL 1N NaOH | ||
| + | |||
| + | 15g (agar for plates) | ||
| + | |||
| + | 1. antibody DNA resuspended in TE buffer, 0.1 μg/μL | ||
| + | |||
| + | 2. 5mL LB inoculated with single colony DH5a pro | ||
| + | |||
| + | 3. Incubated at 37 degrees overnight | ||
Latest revision as of 15:49, 30 July 2008
Antibody/GPCR Fusion
- Consulting meeting with Huimin Zhao (Associate Professor, Chemical and Biomolecular Engineering)
LAB WORK
Receptor Tyrosine Kinase method
E.Coli Media
LB medium, per liter
10g tryptone
5g yeast extract
5g NaCl
1 mL 1N NaOH
15g (agar for plates)
1. antibody DNA resuspended in TE buffer, 0.1 μg/μL
2. 5mL LB inoculated with single colony DH5a pro
3. Incubated at 37 degrees overnight
