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Wisconsin: Lignin Project/17 June 2008
From 2008.igem.org
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== Team Sorbitol == | == Team Sorbitol == | ||
| - | Harvested the chromosomal DNA from the overnight MG1655 cultures. | + | Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells. |
Used this DNA as a template for 2 PCR reactions | Used this DNA as a template for 2 PCR reactions | ||
Revision as of 19:48, 31 July 2008
Team Fungus:
Received primers
Diluted primers
Ran TAQ PCR reaction with Carbon-limited cDNA
Ran PCR product on 1% agrose gel; only ladder visible
Team Sorbitol
Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells.
Used this DNA as a template for 2 PCR reactions
One reaction was to clone out srlD the other was to clone the srl operon.
However when run on a gel no bands were found.
