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- 18:48, 28 October 2008 (diff | hist) Team:Wisconsin/Parts (→Biobricks) (top)
- 18:48, 28 October 2008 (diff | hist) Team:Wisconsin/Parts (→Biobrick)
- 18:48, 28 October 2008 (diff | hist) Team:Wisconsin/Parts (→Biobrick)
- 16:56, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/31 August 2008 (top)
- 16:55, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/30 August 2008 (top)
- 16:54, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/29 August 2008
- 16:53, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/28 August 2008
- 16:52, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/28 August 2008
- 16:51, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/27 August 2008
- 16:50, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/11 August 2008
- 16:48, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/9 August 2008 (top)
- 16:47, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/8 August 2008
- 16:46, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/7 August 2008
- 16:44, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/6 August 2008
- 16:43, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/5 August 2008
- 16:38, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/4 August 2008 (top)
- 16:37, 28 October 2008 (diff | hist) Wisconsin:Lignin Project/4 August 2008
- 20:46, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/28 July 2008 (New page: == Team Sorbitol == Harvested the DNA from these cultures and digested - however they were not the right size so will reperform the ligation)
- 20:45, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/27 July 2008 (New page: == Team Sorbitol == Got several colonies from the transformation - grew them up in liquid culture.)
- 20:44, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/26 July 2008 (New page: == Team Sorbitol == Transformed the ligations into chemically competent DH5a and allowed to grow overnight)
- 20:44, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/25 July 2008 (New page: == Team Sorbitol == Digested the ''srlD'' and pET28a with BamHI and HindIII Extracted the digestion and performed a ligation which ran overnight at 4C)
- 20:42, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/23 July 2008
- 20:41, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/23 July 2008 (New page: == Team Sorbitol == Sucessfully cloned out the ''srl'' operon)
- 20:40, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/18 July 2008 (New page: == Team Sorbitol == Froze down 3 400ul samples of RL257, MG1655, and JW3890 each containing pBAD30-srlD. They were then stored at -80. Primers were then designed and ordered to insert '...)
- 20:38, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/17 July 2008 (New page: == Team Sorbitol == Grew up 2mL cultures of the successful transformations from 7/15. Also designed several primers that will be used for biobricking ''srlD'' We ordered these primers a...)
- 20:37, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/15 July 2008 (New page: == Team Sorbitol == All the transformations worked correctly)
- 20:35, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/14 July 2008 (New page: == Team Sorbitol == Met with Dr. Pfleger and learned the specifics of biobricking. Designed a procedure to biobrick our genes. Following Weibel Lab protocols transformed pBAD30-srlD int...)
- 20:31, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/11 July 2008 (New page: == Team Sorbitol == Froze down 3 400ul samples of the overnight DH5a-pBAD30-Srld E.coli for future use. Harvested the DNA from the pBAD30, PBAD30-srlD, and pBAD18 using a miniprep kit. ...)
- 20:29, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/10 July 2008
- 20:28, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/10 July 2008
- 20:25, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/10 July 2008 (New page: == Team Sorbitol ==)
- 20:24, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/9 July 2008 (New page: == Team Sorbitol == The sequencing was cleaned with Agencourt CleanSEQ dye-Terminator removal). It was then taken to the UW-biotech center for sequencing. Attended the Wisconsin iGEM te...)
- 20:23, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/8 July 2008 (New page: == Team Sorbitol == Harvested the DNA from the overnight colonies. Used this DNA in a PCR sequencing reaction. The product was wrapped in foil (to keep out the light) and stored at 4C o...)
- 20:22, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/7 July 2008 (New page: == Team Sorbitol == The 10 colonies were harvested with a Qiagen Miniprep Kit and the DNA was digested with Xba1 and HindIII for 1 hour at 37C. The digestion was then run on an agarose g...)
- 20:19, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/6 July 2008 (New page: == Team Sorbitol == Took out the transformed plates - all grew! Started liquid cultures of 10 colonies to verify the transformation was correct)
- 20:18, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/28 May 2008
- 20:18, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/3 July 2008 (New page: == Team Sorbitol == Created SOC media for transformations. Transformed each of the ligations (as well as controls) into our chemically competent stocks of DH5a. To do this we followed th...)
- 20:16, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/2 July 2008
- 20:16, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/2 July 2008
- 20:15, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/2 July 2008 (New page: == Team Sorbitol == The pBAD30 was harvested using a Qiagen Miniprep kit and digested as described before. We were able to extract enough plasmid DNA to perform a ligation. We then set ...)
- 20:13, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/1 July 2008 (New page: == Team Sorbitol == The overnight PCR reaction failed. Used the purified PCR product from yesterday to set up a digestion (along with pBAD30) as described before. The digestion was veri...)
- 20:10, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/30 June 2008 (New page: == Team Sorbitol == Repeated the successful PCR reaction from before. This time we obtained successful band for both ''srlD'' 700bp and the ''srl'' operon 2700bp These products were puri...)
- 20:07, 31 July 2008 (diff | hist) N Wisconsin: Lignin Project/29 June 2008 (New page: == Team Sorbitol == Began a 10mL culture of DH5a with pBAD30 in order to maximize our amount of DNA obtained after a digestion.)
- 20:06, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/27 June 2008
- 20:03, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/26 June 2008
- 20:00, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/24 June 2008
- 19:59, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/25 June 2008
- 19:57, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/24 June 2008
- 19:56, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/23 June 2008
- 19:54, 31 July 2008 (diff | hist) Wisconsin: Lignin Project/19 June 2008
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