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Wisconsin: Lignin Project/27 June 2008
From 2008.igem.org
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(New page: '''Team Fungus:''' <br> Plate with 1:3 insert to vector ratio had 2 colonies<br> Plate with 0:1 vector had 1 colony<br> Rest had zero colonies<br> We streaked and plated the two colonies f...) |
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| - | + | == Team Sorbitol == | |
| + | Checked the PCR product from last night on a gel and found several bands to work again (700bp). | ||
| + | |||
| + | The product and pBAD30 were digested with Xba1 and HindIII restriction enzymes. | ||
| + | |||
| + | They were verified to work on a gel and extracted using a Qiagen Gel Extration Kit. | ||
| + | |||
| + | Unfortunately not enough DNA was obtained to perform a ligation. | ||
Revision as of 20:06, 31 July 2008
Team Fungus:
Plate with 1:3 insert to vector ratio had 2 colonies
Plate with 0:1 vector had 1 colony
Rest had zero colonies
We streaked and plated the two colonies from the 1:3 onto two new LB + Kan plates
Made more LB + Kan plates
Team Sorbitol
Checked the PCR product from last night on a gel and found several bands to work again (700bp).
The product and pBAD30 were digested with Xba1 and HindIII restriction enzymes.
They were verified to work on a gel and extracted using a Qiagen Gel Extration Kit.
Unfortunately not enough DNA was obtained to perform a ligation.
