Team:Missouri Miners

From 2008.igem.org

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Revision as of 01:04, 1 August 2008

This is a template page. READ THESE INSTRUCTIONS.

You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.

You MUST have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your Team:Example namespace.

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.

The purpose of this research is to use recombinant technology to culture yeast cells capable of determining the concentration of ethanol and using these cells to construct an ethanol sensor. Metabolic pathways exist for the metabolism of methanol and ethanol within some species of the Pichia taxa to include the yeast of our interest, Pichia pastoris. Alcohol oxidase (AO) appears to be the first and major enzyme produced in the methanol metabolic pathway of P. pastoris. However, if both ethanol and methanol are present, P. pastoris will utilize the ethanol before consuming the methanol. Consequently, the AOXI gene will not be expressed to produce the AO enzyme until the ethanol has been consumed.

Fusing the AOXI gene promoter with the DNA sequence encoding a fluorescent protein will allow the expression of the AOXI gene to be detected. In supplying the yeast cells with ethanol and methanol simultaneously, the cells will produce the fluorescent protein once the ethanol is utilized. The concentration of ethanol can then be determined by measuring the time before fluorescence is detected.

Your team picture

Team Example 2

Home	The Team	The Project	Parts Submitted to the Registry	Modeling	Notebook

(Or you can choose different headings. But you must have a team page, a project page, and a notebook page.)

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-''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+The purpose of this research is to use recombinant technology to culture yeast cells capable of determining the concentration of ethanol and using these cells to construct an ethanol sensor.  Metabolic pathways exist for the metabolism of methanol and ethanol within some species of the Pichia taxa to include the yeast of our interest, ''Pichia pastoris''.  Alcohol oxidase (AO) appears to be the first and major enzyme produced in the methanol metabolic pathway of ''P. pastoris''. However, if both ethanol and methanol are present, P. pastoris will utilize the ethanol before consuming the methanol.  Consequently, the AOXI gene will not be expressed to produce the AO enzyme until the ethanol has been consumed.
+Fusing the AOXI gene promoter with the DNA sequence encoding a fluorescent protein will allow the expression of the AOXI gene to be detected.  In supplying the yeast cells with ethanol and methanol simultaneously, the cells will produce the fluorescent protein once the ethanol is utilized.  The concentration of ethanol can then be determined by measuring the time before fluorescence is detected.
+''
 |[[Image:Team.png|right|frame|Your team picture]]
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