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Team:Bologna/Protocols
From 2008.igem.org
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# Puncture a hole through the foil with a pipette tip (wash it everytime with bleach-distilled water-EOH 95%) into the well that corresponds to the Biobrick™-standard part that you want. | # Puncture a hole through the foil with a pipette tip (wash it everytime with bleach-distilled water-EOH 95%) into the well that corresponds to the Biobrick™-standard part that you want. | ||
| - | # Add | + | # Add 5 μl of TE buffer. |
| + | # Rest for 20 minutes at 50 °C | ||
| + | |||
== Transformation == | == Transformation == | ||
== Inoculation == | == Inoculation == | ||
Revision as of 16:27, 4 August 2008
| HOME | THE PROJECT | THE TEAM | PARTS SUBMITTED TO THE REGISTRY | MODELING | NOTEBOOK |
|---|
Contents |
Plates preparation
- Autoclave LB medium with 2% agar.
- Cool at 50 °C to prevent agar polymerization.
- Before preparing plates add antibiotic (Ampicillin 1000x [ ] or Kanamicin 200x [ ]).
- Put about 20ml of medium per plate.
- Leave it solidify and store at 4°C.
Biobricks amplification
- Puncture a hole through the foil with a pipette tip (wash it everytime with bleach-distilled water-EOH 95%) into the well that corresponds to the Biobrick™-standard part that you want.
- Add 5 μl of TE buffer.
- Rest for 20 minutes at 50 °C
