Team:Bologna/Parts

From 2008.igem.org

Logo1a.gifTestata dx.jpg
HOME PROJECT TEAM SOFTWARE MODELING WET LAB LAB-BOOK SUBMITTED PARTS BIOSAFETY AND PROTOCOLS


Composites

The table below shows all the composites that were obtained and use to build up our final device. For each part, it ispossible to find:

-the link to the Registry in the second column ;

-part components, in the third column ;

-in the fourth column the plasmid the part has been cloned in;

-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the sixth column cell vitality after transformation;

-in the seventh column fluo data for each part.


Igem Code Fragment Plasmid Length (bp) Vitality Fluo
1
K079015 LacY transporter protein from E. coli (for the improvement of the registry) pSB1AK3 (HC) 1254
Yes
-
2
K079017 Lac symmetric - operator library member GENEART pGA18 20
Yes
-
3
K079018 Lac 1 - operator library member GENEART pGA18 21
Yes
-
4
K079019 Lac 2 - operator library member GENEART pGA18 21
Yes
-
5
K079036 Tet O operator library member GENEART pMA 15
Yes
-
6
K079037 TetO-4C - operator library member GENEART pMA 15
Yes
-
7
K079038 TetO-wt/4C5G - operator library member GENEART pMA 15
Yes
-
8
K079039 LexA 1 - operator library member GENEART pMA 16
Yes
-
9
K079040 LexA 2 - opeartor library member GENEART pMA 16
Yes
-
10
K079041 Lambda OR1 - operator library member GENEART pMA 17
Yes
-
11
K079042 Lambda OR2 - operator library member GENEART pMA 17
Yes
-
12
K079043 Lambda OR3 - operator library member GENEART pMA 17
Yes
-
13
K079045 Lac operator library GENEART pGA18 78
Yes
-
14
K079046 Tet operator library GENEART pMA 61
Yes
-
15
K079047 Lambda operator library GENEART pMA 67
Yes
-
16
K079048 LexA operator library GENEART pMA 40
Yes
-
17
K079020 GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein pSB1A2 (HC) 2165
Yes
18
K079026 GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter pSB1A2 (HC) 2116
Yes
19
K079029 GFP expression controlled by LacI repressor under the BBa_J23114 promoter pSB1A2 (HC) 2501
Yes
20
K079049 GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator pSB1A2 (HC) 981
Yes
-
21
K079050 GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator pSB1A2 (HC) 981
Yes
22
K079023 Auto-regulated J23118 constitutive promoter and Lac 2 operator pSB1A2 (HC) 1243
Yes
-
23
K079024 LacI repressor under the control of the J23118 constitutive promoter and Lac 1 operator pSB1A2 (HC) 1243
Yes
-
24
K079030 GFP reporter protein under the control of the BBa_23105 constitutive promoter pSB1A2 (HC) 957
Yes
25
K079031 GFP reporter protein under the control of the BBa_J23118 promoter pSB1A2 (HC) 957
Yes
26
K079032 GFP reporter protein under the control of the BBa_J23100 constitutive promoter pSB1A2 (HC) 957
Yes


Up