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Team:KULeuven/4 August 2008
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* Competent cells were inoculated and prepared for elektroporation with the folowing ligation products: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015, P1010+B0015, C0060+B0015, C0062+B0015, R0084+B0032,... The elektroporation was performed and hopefully we will have transformed cells tomorrow. | * Competent cells were inoculated and prepared for elektroporation with the folowing ligation products: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015, P1010+B0015, C0060+B0015, C0062+B0015, R0084+B0032,... The elektroporation was performed and hopefully we will have transformed cells tomorrow. | ||
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| + | * We made electrocompetent cells and tested them with pUC. Tomorrow we'll see if they're any good. A glycerolstock of the cells has been stored in the -80° freezer. | ||
=== Dry Lab === | === Dry Lab === | ||
Revision as of 06:39, 5 August 2008
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Contents |
Lab Work
Wet Lab
- Competent cells were inoculated and prepared for elektroporation with the folowing ligation products: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015, P1010+B0015, C0060+B0015, C0062+B0015, R0084+B0032,... The elektroporation was performed and hopefully we will have transformed cells tomorrow.
- We made electrocompetent cells and tested them with pUC. Tomorrow we'll see if they're any good. A glycerolstock of the cells has been stored in the -80° freezer.
