Team:KULeuven/4 August 2008

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• The Team
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  Summary Components

  Input Output Filter InverTimer Reset Cell Death Memory

  Safety End Evaluation Literature Brainstorm
• Ethics
  Introduction Three A's

  Defining the three A's Developments in modern physics Developments in biology

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  Defining synthetic biology A framework Concerns Issues The need for answers

  Biological Robotics

  KULeuven iGEM 2008 project Biological robotics

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• Modeling
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  Output Filter InverTimer Reset Cell Death Memory

  Full Model Sensitivity Analysis Multi-cell Model Diffusion
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  GFP (LVA-tag) Other

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Lab Work

Wet Lab

Competent cells were inoculated and prepared for electroporation with the folowing ligation products: [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032], [http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032], [http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_P1010 P1010]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_J23100 J23100]+[http://partsregistry.org/Part:BBa_B0032 B0032]. The electroporation was performed and hopefully we will have transformed cells tomorrow.

We continued to make the GFP-LVA Biobrick ([http://partsregistry.org/Part:BBa_K145015 K145015]). The PCR product was put on gel and purified. It gave nice results, so the PCR worked well. Tomorrow we will cut it with EcoRI and SpeI.

We made electrocompetent cells and tested them with pUC. Tomorrow we'll see if they're any good. A glycerolstock of the cells has been stored in the -80° freezer.

Dry Lab

Dries and Jonas started work on the Dr. Coli logo.

Modeling

We've made a phase plot of the memory to prove the existence of two distinct basins of attraction. Nick has looked into multi-cell interactions (multi-agent systems) a bit. Conclusion is that it might be a bit out of our scope.

Also decided to ignore cell division for now. The memory appears sufficiently stable to be passed on to the cells offspring. We suspect that the quantitites of our proteins and mRNA's should remain relatively stable.

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