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Team:KULeuven/4 August 2008
From 2008.igem.org
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| - | * Competent cells were inoculated and prepared for | + | * Competent cells were inoculated and prepared for electroporation with the folowing ligation products: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015, P1010+B0015, C0060+B0015, C0062+B0015, R0084+B0032, J23100+B0032. The electroporation was performed and hopefully we will have transformed cells tomorrow. |
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| + | * We continued to make the GFP-LVA Biobrick. The PCR product was put on gel and purified. It gave nice results,so the PCR worked well. Tomorrow we will cut it with ''Eco''RI and ''Spe''I. | ||
* We made electrocompetent cells and tested them with pUC. Tomorrow we'll see if they're any good. A glycerolstock of the cells has been stored in the -80° freezer. | * We made electrocompetent cells and tested them with pUC. Tomorrow we'll see if they're any good. A glycerolstock of the cells has been stored in the -80° freezer. | ||
Revision as of 08:54, 5 August 2008
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Contents |
Lab Work
Wet Lab
- Competent cells were inoculated and prepared for electroporation with the folowing ligation products: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015, P1010+B0015, C0060+B0015, C0062+B0015, R0084+B0032, J23100+B0032. The electroporation was performed and hopefully we will have transformed cells tomorrow.
- We continued to make the GFP-LVA Biobrick. The PCR product was put on gel and purified. It gave nice results,so the PCR worked well. Tomorrow we will cut it with EcoRI and SpeI.
- We made electrocompetent cells and tested them with pUC. Tomorrow we'll see if they're any good. A glycerolstock of the cells has been stored in the -80° freezer.
