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Team:Hawaii/Notebook/2008-08- 4
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid Prep=== :<strong>Margaret</strong> :* I14032, oriT ===Quantification of all my Parts with Gel=== :<strong>Mar...) |
(→Re-streak Library) |
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:* pSB1A7, L51020, psb3k3, psb1a3, oriT, psb1a2, db3.1 strain, i14032 | :* pSB1A7, L51020, psb3k3, psb1a3, oriT, psb1a2, db3.1 strain, i14032 | ||
| + | |||
| + | ===Construction of p+r, g+t=== | ||
| + | :<strong>Grace</strong> | ||
| + | |||
| + | :* Gel purified nir1, GFPf1, GFP, E0240, I14032, slr1, slr2, pilA PCR products | ||
| + | :* RE digest | ||
| + | ::* nir, GFP, GFPf, I14032 digested with EcoRI and SpeI | ||
| + | ::* E0240 digested with EcoRI and PstI | ||
| + | ::* slr1, slr2, pilA digested with PstI and XbaI | ||
| + | :* Ran RE digests on gel | ||
| + | ::* Incomplete digestion of nir, slr1, slr2, pilA | ||
| + | |||
| + | :<strong>Krystle</strong> | ||
| + | |||
| + | :* Gel purified RE digests of nir1, GFPf1, GFP, E0240, I14032, slr1, slr2, pilA | ||
| + | :* Ligated: | ||
| + | ::* nir + B0030 | ||
| + | ::* I14032 + B0030 | ||
| + | ::* GFP + B0015 | ||
| + | ::* GFPf + B0015 | ||
| + | |||
| + | === PCR=== | ||
| + | :<strong>Grace</strong> | ||
| + | |||
| + | |||
| + | :* Redid Saturday's PCR of BB-pRl1383a and pRL1383a alongside test of PCR contamination | ||
| + | ::* Ran on 2.5% gel for 1 hour at 95V | ||
| + | :::* Gel doesn't match that from Saturday | ||
| + | :* Redid PCR again to verify Saturday/today's PCR | ||
== Drylab Work == | == Drylab Work == | ||
Revision as of 19:19, 5 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid Prep
- Margaret
- I14032, oriT
Quantification of all my Parts with Gel
- Margaret
- I14032, oriT, L51020, pSB1A7, B015, B0034
PCR
- Margaret
- Rep, oriV, verification of I14032, oriT(just to make sure)
Transformation
- Margaret
- pSMC121 (we were running out of template)
Re-streak Library
- Margaret
- pSB1A7, L51020, psb3k3, psb1a3, oriT, psb1a2, db3.1 strain, i14032
Construction of p+r, g+t
- Grace
- Gel purified nir1, GFPf1, GFP, E0240, I14032, slr1, slr2, pilA PCR products
- RE digest
- nir, GFP, GFPf, I14032 digested with EcoRI and SpeI
- E0240 digested with EcoRI and PstI
- slr1, slr2, pilA digested with PstI and XbaI
- Ran RE digests on gel
- Incomplete digestion of nir, slr1, slr2, pilA
- Krystle
- Gel purified RE digests of nir1, GFPf1, GFP, E0240, I14032, slr1, slr2, pilA
- Ligated:
- nir + B0030
- I14032 + B0030
- GFP + B0015
- GFPf + B0015
PCR
- Grace
- Redid Saturday's PCR of BB-pRl1383a and pRL1383a alongside test of PCR contamination
- Ran on 2.5% gel for 1 hour at 95V
- Gel doesn't match that from Saturday
- Redid PCR again to verify Saturday/today's PCR
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
