Team:Minnesota/PartsComparator

From 2008.igem.org

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|The Minnesota iGEM '08 team consists  of groups working on two different projects. One team is located primarily in Minneapolis and is designing a comparator for their project. The other team is based in St. Paul and is working on a time bomb.
 
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'''Team Comparator'''
 
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|Control systems are an integral component of almost all aspects of life. Whether it is in industrial, biological, or chemical applications, controllers provide a way to keep systems functioning properly. A vital part of any control system is the comparator. This component compares a set point value and a measured value, and determines which is larger. It then sends the appropriate signal to the controller, which reacts to bring the system back to the set point. In typical applications, the controller equipment is electronic. However, our team set out to create a comparator using only genetic components. This comparator could potentially be used as part of a new, solely biological control system that could be used to treat many diseases afflicting humans, for example diabetes. This comparator could compare the blood sugar of a patient to what it should be, and send this result to a control system that could compensate, for example by changing the levels of insulin.
 
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In order to undertake this task, a system involving six genes was designed. For our system, the two inputs (one representing the set point and one representing the measured value) are IPTG and ATC. These inputs will activate the transcription of the LacI and TetR proteins, and set in motion the rest of the system to produce the outputs. Depending on the amounts of the two inducer molecules added to the system, either green fluorescent protein (GFP) or red fluorescent protein(RFP) will be produced. The actual design of the system can be seen below. In order to complete this project, a total of six genes will have to be cloned into plasmids, and two new BioBrick parts will be produced. One will be a TetR and p22-MNT dual-repressed promoter, and the other will be a LacI and lambdaphage cI dual-repressed promoter. We will also build mathematical models and conduct computer simulations that will help with the designs. This project will pave the way for other parts of a true genetic PID controller to be produced, which could be an exciting scientific development in the near future.
 
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|[[Image:Team_Pic_Minnesota.jpg|300px|right|thumb|Team Comparator]]
 
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<!--- The Mission, Experiments --->
 
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{| style="color:gold;background-color:#800000;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="90%" align="center"
{| style="color:gold;background-color:#800000;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="90%" align="center"
!align="center"|[[Team:Minnesota|<font color="gold">Minnesota Home</font>]]
!align="center"|[[Team:Minnesota|<font color="gold">Minnesota Home</font>]]
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===Note===
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===Parts Submitted===
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here.  This is not necessary but it may be a nice list to keep track of.
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|BBa_K101000 - Dual-Repressed Promoter for p22 mnt and TetR
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|BBa_K101001 - Dual-Repressed Promoter for LacI and LambdacI
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|BBa_K101002 - Dual-Repressed Promoter for p22 cII and TetR
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|BBa_K101003 - TetR promoter, RBS, Lambda cI gene, terminator
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|BBa_K101004 - LacI promoter, RBS, p22 mnt gene, terminator
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|BBa_K101005 - LacI/Lamsda cI promoter, RBS, GFP, terminator
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|BBa_K101006 - TetR/p2 mnt promoter, RBS, RFP, terminator
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|BBa_K101007 - TetR/p2 cII promoter, RBS, RFP, terminator
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| BBa_K101008 - Whole construct of comparator
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|BBa_K101009 - Dual-Repressed Promoter for p22 mnt and TetR with RBS
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|BBa_K101010 - Dual-Repressed Promoter for LacI and LambdacI with RBS
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|BBa_K101011 - Dual-Repressed Promoter for p22 cII and TetR with RBS
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Latest revision as of 02:07, 8 August 2008

Minnesota Home Team Comparator Home The Team The Project Parts Submitted to the Registry Modeling Notebook


Parts Submitted

BBa_K101000 - Dual-Repressed Promoter for p22 mnt and TetR
BBa_K101001 - Dual-Repressed Promoter for LacI and LambdacI
BBa_K101002 - Dual-Repressed Promoter for p22 cII and TetR
BBa_K101003 - TetR promoter, RBS, Lambda cI gene, terminator
BBa_K101004 - LacI promoter, RBS, p22 mnt gene, terminator
BBa_K101005 - LacI/Lamsda cI promoter, RBS, GFP, terminator
BBa_K101006 - TetR/p2 mnt promoter, RBS, RFP, terminator
BBa_K101007 - TetR/p2 cII promoter, RBS, RFP, terminator
BBa_K101008 - Whole construct of comparator
BBa_K101009 - Dual-Repressed Promoter for p22 mnt and TetR with RBS
BBa_K101010 - Dual-Repressed Promoter for LacI and LambdacI with RBS
BBa_K101011 - Dual-Repressed Promoter for p22 cII and TetR with RBS
Retrieved from "http://2008.igem.org/Team:Minnesota/PartsComparator"