Team:Bologna/Notebook

From 2008.igem.org

(Difference between revisions)
Line 19: Line 19:
<br>
<br>
-
== [[Team:Bologna/Protocols|PROTOCOLS]] ==
+
= [[Team:Bologna/Protocols|PROTOCOLS]] =
* [[Team:Bologna/Protocols#Plates_preparation|Plates preparation]]
* [[Team:Bologna/Protocols#Plates_preparation|Plates preparation]]
* [[Team:Bologna/Protocols#Amplification|Amplification]]
* [[Team:Bologna/Protocols#Amplification|Amplification]]
Line 38: Line 38:
-
== WEEK 1: from 07/21/08 to 07/27/08 ==
+
= WEEK 1: from 07/21/08 to 07/27/08 =
# Enzymatic digestion of: C0051 E\S, LACI* X\P.
# Enzymatic digestion of: C0051 E\S, LACI* X\P.
Line 45: Line 45:
-
== WEEK 2: from 07/28/08 to 08/03/08 ==
+
= WEEK 2: from 07/28/08 to 08/03/08 =
# Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10) <br>
# Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10) <br>
Line 51: Line 51:
-
== WEEK 3: from 08/04/08 to 08/10/08 ==
+
= WEEK 3: from 08/04/08 to 08/10/08 =
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>

Revision as of 15:11, 8 August 2008

Testata sx.jpg Testata dx.jpg
HOME THE PROJECT THE TEAM PARTS SUBMITTED TO THE REGISTRY MODELING NOTEBOOK


Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.


Contents

PROTOCOLS


WEEK 1: from 07/21/08 to 07/27/08

  1. Enzymatic digestion of: C0051 E\S, LACI* X\P.
  2. Gel preparation , electrophoretic run.
  3. Amplification of: TETR , PUV, PRECA.


WEEK 2: from 07/28/08 to 08/03/08

  1. Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10)
  2. Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240


WEEK 3: from 08/04/08 to 08/10/08