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Wisconsin: Lignin Project/17 June 2008
From 2008.igem.org
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| + | |'''Team Sorbitol:''' | ||
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| + | Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells.<br> | ||
| + | Used this DNA as a template for 2 PCR reactions.<br> | ||
| + | One reaction was to clone out ''srlD'' the other was to clone the ''srl'' operon.<br> | ||
| + | However when run on a gel no bands were found.<br> | ||
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'''Team Fungus:''' <br> | '''Team Fungus:''' <br> | ||
Received primers <br> | Received primers <br> | ||
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Ran TAQ PCR reaction with Carbon-limited cDNA <br> | Ran TAQ PCR reaction with Carbon-limited cDNA <br> | ||
Ran PCR product on 1% agrose gel; only ladder visible <br> | Ran PCR product on 1% agrose gel; only ladder visible <br> | ||
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Latest revision as of 15:15, 8 August 2008
| Team Sorbitol:
Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells. Team Fungus: |
