Team:Montreal/Notebook

From 2008.igem.org

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Revision as of 02:34, 29 May 2008

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Home

The Team

The Project

Parts Submitted to the Registry

Modeling

Notebook

Lab Progress

May 22nd, 2008: - Prepared TOP10 Competent cells for eventual transformation. - Performed Mini-prep on Reporter+ Cells - Performed Digest and Gel on Reporter plasmid extract -- no DNA present, suggest follow-up maxi-prep

May 25th, 2008: - Diluted Reporter cells 1/1000 with 5ul Kan/mL culture for 16h incubation at 5:30pm. To be used for Maxiprep at 9:30am-1:30pm.

May 28th, 2008: - Ran gel on Elowitz reporter DNA cut with EcoR1; 2 bands - 0.7 kb and 2.0 kb, confirms identity of reporter DNA. - Seeded syn-I and J-40001 into amp/kan LB and kan LB.

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 ==Lab Progress==